Experimental study of histological changes in vascular loops according to the duration of the postoperative period: Application in reconstructive microsurgery

Authors

  • Renata Gregorio Paulos Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Bruno Alves Rudelli Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Renee Zon Filippe Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Gustavo Bispo dos Santos Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Ana Abarca Herrera Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Andre Araujo Ribeiro Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Marcelo Rosa de Rezende Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Teng Hsiang-Wei Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)
  • Rames Mattar-Jr Universidade de São Paulo; Hospital das Clinicas HCFMUSP, Faculdade de Medicina; Departamento de Ortopedia e Traumatologia, Instituto de Ortopedia e Traumatologia (IOT)

DOI:

https://doi.org/10.6061/clinics/2017(09)03

Keywords:

Vascular Loop, Intimal Hyperplasia, Vascular Remodeling

Abstract

OBJECTIVES: To analyze the histological changes observed in venous grafts subjected to arterial blood flow as a function of the duration of the postoperative period to optimize their use in free flap reconstructions. METHOD: Twenty-five rats (7 females and 18 males) underwent surgery. Surgeries were performed on one animal per week. Five weeks after the first surgery, the same five animals were subjected to an additional surgery to assess the presence or absence of blood flow through the vascular loop, and samples were collected for histological analysis. This cycle was performed five times. RESULTS: Of the rats euthanized four to five weeks after the first surgery, no blood flow was observed through the graft in 80% of the cases. In the group euthanized three weeks after the first surgery, no blood flow was observed in 20% of the cases. In the groups euthanized one to two weeks after the first surgery, blood flow through the vascular loop was observed in all animals. Moreover, intimal proliferation tended to increase with the duration of the postoperative period. Two weeks after surgery, intimal proliferation increased slightly, whereas strong intimal proliferation was observed in all rats evaluated five weeks after surgery. CONCLUSION: Intimal proliferation was the most significant change noted in venous grafts as a function of the duration of the postoperative period and was directly correlated with graft occlusion. In cases in which vascular loops are required during free flap reconstruction, both procedures should preferably be performed during the same surgery.

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Published

2017-10-01

Issue

Section

Clinical Sciences

How to Cite

Paulos, R. G., Rudelli, B. A., Filippe, R. Z., dos Santos, G. B., Herrera, A. A., Ribeiro, A. A., de Rezende, M. R., Hsiang-Wei, T., & Mattar-Jr, R. (2017). Experimental study of histological changes in vascular loops according to the duration of the postoperative period: Application in reconstructive microsurgery. Clinics, 72(9), 538-542. https://doi.org/10.6061/clinics/2017(09)03