Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis

Authors

  • Bhagath Kumar Potu Manipal University; Kasturba Medical College; Department of Anatomy
  • Muddanna S Rao Manipal University; Kasturba Medical College; Department of Anatomy
  • N Gopalan Kutty Manipal College of Pharmaceutical Sciences; Department of Pharmacology
  • Kumar MR Bhat Manipal University; Kasturba Medical College; Department of Anatomy
  • Mallikarjuna Rao Chamallamudi Manipal College of Pharmaceutical Sciences; Department of Pharmacology
  • Soubhagya Ranjan Nayak Manipal University; Kasturba Medical College; Department of Anatomy

DOI:

https://doi.org/10.1590/S1807-59322008000600018

Keywords:

Estrogens, Phytoestrogens, Ossification, Alizarin red-Alcian blue

Abstract

OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. RESULTS: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group. CONCLUSION: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

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Published

2008-01-01

Issue

Section

Basic Research

How to Cite

Potu, B. K., Rao, M. S., Kutty, N. G., Bhat, K. M., Chamallamudi, M. R., & Nayak, S. R. (2008). Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis . Clinics, 63(6), 815-820. https://doi.org/10.1590/S1807-59322008000600018