Does minor histocompatibility antigen HA-1 disparity affect the occurrence of graft-versus-host disease in tunisian recipients of hematopoietic stem cells?

Authors

  • Mohamed Hichem Sellami National Blood Transfusion Centre of Tunis; HLA-Typing Department
  • Lamia Torjemane National Blood Transfusion Centre of Tunis; HLA-Typing Department
  • Alejandro Espadas de Arias Ospedale Maggiore Policlinico di Milano; Department of Regenerative Medicine
  • Houda Kaabi National Blood Transfusion Centre of Tunis; HLA-Typing Department
  • Saloua Ladeb National Blood Transfusion Centre of Tunis; HLA-Typing Department
  • Francesca Poli Ospedale Maggiore Policlinico di Milano; Department of Regenerative Medicine
  • Tarek Ben Othmane National Blood Transfusion Centre of Tunis; HLA-Typing Department
  • Slama Hmida National Blood Transfusion Centre of Tunis; HLA-Typing Department

DOI:

https://doi.org/10.1590/S1807-59322010001100007

Keywords:

Hematopoietic stem cell transplantation, Graft-versus-host disease, Minor histocompatibility antigens, HA-1, HLA-A, Tunisian population

Abstract

INTRODUCTION: Minor histocompatibility antigen HA-1 (MiHAg-HA-1) disparity between a patient and his or her human leukocyte antigen (HLA) genoidentical donor has been widely associated with an increased risk of graft-versus-host disease following allogeneic hematopoietic stem cell transplantation. OBJECTIVE: To examine the effect of HA-1 disparity on the incidence of both acute and chronic graft-versus-host disease in Tunisian recipients of hematopoietic stem cells. METHODS: A total of 60 patients and their 60 respective sibling hematopoietic stem cell donors were enrolled in this study. All patients prophylactically received cyclosporine A and/or methotrexate for graft-versus-host disease. An HA-1 genotyping assay was performed with the SSP-PCR method, and HLA-A*0201- and/or HLA-A*0206-positive samples were identified using the Luminex HLA typing method. RESULTS: The Luminex HLA typing assay showed that 54 patients were positive for either the HLA-A*0201 or HLA-A*0206 alleles. Among these cases, six pairs were mismatched for MiHAg-HA-1. Both acute and chronic graft-versus-host disease occurred in four mismatched patients (Fisher's p-values were 0.044 and 0.170, respectively). A univariate logistic regression model analysis showed that only acute graft-versus-host disease may be affected by recipient MiHAg-HA-1 disparity (p: 0.041, OR: 6.727), while chronic graft-versus-host disease correlates with both age and recipient/donor sex mismatch (p: 0.014, OR: 8.556 and p: 0.033, OR: 8.664, respectively). CONCLUSION: Our findings support previously reported data suggesting a significant association between HA-1 disparity and the risk of acute graft-versus-host disease following hematopoietic stem cell transplantation.

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Published

2010-01-01

Issue

Section

Clinical Sciences

How to Cite

Sellami, M. H., Torjemane, L., Arias, A. E. de, Kaabi, H., Ladeb, S., Poli, F., Othmane, T. B., & Hmida, S. (2010). Does minor histocompatibility antigen HA-1 disparity affect the occurrence of graft-versus-host disease in tunisian recipients of hematopoietic stem cells? . Clinics, 65(11), 1099-1103. https://doi.org/10.1590/S1807-59322010001100007