Long non-coding RNA KCNQ1 overlapping transcript 1 promotes the progression of esophageal squamous cell carcinoma by adsorbing microRNA-133b

Authors

  • Haitao Xu Binzhou Medical University Hospital. Department of Thoracic Surgery
  • Jing Miao Binzhou People’s Hospital. Department of Pediatrics
  • Shuai Liu Binzhou Medical University Hospital. Department of Thoracic Surgery
  • Hongjian Liu Binzhou Medical University Hospital. Department of Thoracic Surgery
  • Lianguo Zhang Binzhou Medical University Hospital. Department of Thoracic Surgery
  • Qingguang Zhang Binzhou Medical University Hospital. Department of Thoracic Surgery

DOI:

https://doi.org/10.6061/clinics/2021/e2175

Keywords:

KCNQ1OT1, miR-133b, EGFR, PI3K/AKT, ESCC

Abstract

OBJECTIVE: The long non-coding RNA (lncRNA) KCNQ1 overlapping transcript 1 (KCNQ1OT1) exerts vital regulatory functions in diverse tumors. However, the biological function of KCNQ1OT1 in esophageal squamous cell carcinoma (ESCC) remains unclear. METHODS: KCNQ1OT1 expression was detected in ESCC tissues using quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, apoptosis, migration, and invasion were detected by the CCK-8 assay, EdU assay, flow cytometry analysis, and Transwell experiments, respectively. Bioinformatics analysis, luciferase reporter experiments, and RNA immunoprecipitation assays were used to predict and validate the regulatory relationships between KCNQ1OT1, microRNA-133b (miR-133b) and epidermal growth factor receptor (EGFR). RESULTS: KCNQ1OT1 expression was remarkably upregulated in ESCC tissues and cell lines. Overexpression of KCNQ1OT1 markedly promoted ESCC cell proliferation, migration, and invasion and enhanced the expression of N-cadherin, MMP-2, and MMP-9, but inhibited apoptosis and E-cadherin expression in ESCC cell lines; KCNQ1OT1 knockdown exerted the opposite effects. KCNQ1OT1 could directly bind to miR-133b and suppress its expression, and miR-133b reversed the effects of KCNQ1OT1 overexpression in ESCC cells. MiR-133b reduced the expression of epidermal growth factor receptor (EGFR); further, KCNQ1OT1 activated the phosphatidylinositol 3-kinase/AKT serine/threonine kinase 1 (PI3K/AKT) signaling pathway by repressing miR-133b repression and indirectly upregulating EGFR. KCNQ1OT1 expression was positively correlated with EGFR mRNA expression and negatively correlated with miR-133b expression. CONCLUSION: KCNQ1OT1 facilitates ESCC progression by sponging miR-133b and activating the EGFR/PI3K/AKT pathway.

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Published

2021-11-09

Issue

Section

Original Articles

How to Cite

Xu, H. ., Miao, J. ., Liu, S. ., Liu, H. ., Zhang, L. ., & Zhang, Q. . (2021). Long non-coding RNA KCNQ1 overlapping transcript 1 promotes the progression of esophageal squamous cell carcinoma by adsorbing microRNA-133b. Clinics, 76, e2175. https://doi.org/10.6061/clinics/2021/e2175