Curcumin, but not Prima-1, decreased tumor cell proliferation in the syngeneic murine orthotopic bladder tumor model

Authors

  • Fábio T. Watanabe Universidade de São Paulo; Faculdade de Medicina; Department of Urology
  • Daher C. Chade Universidade de São Paulo; Faculdade de Medicina; Instituto do Câncer do Estado de São Paulo; Department of Urology
  • Sabrina T. Reis Universidade de São Paulo; Faculdade de Medicina; Department of Urology
  • Camila Piantino Universidade de São Paulo; Faculdade de Medicina; Department of Urology
  • Marcos Francisco Dall’ Oglio Universidade de São Paulo; Faculdade de Medicina; Instituto do Câncer do Estado de São Paulo; Department of Urology
  • Miguel Srougi Universidade de São Paulo; Faculdade de Medicina; Department of Urology
  • Katia R. M. Leite Universidade de São Paulo; Faculdade de Medicina; Department of Urology

DOI:

https://doi.org/10.1590/S1807-59322011001200019

Keywords:

Bladder cancer, Treatment, Curcumin, Prima-1, Apoptosis

Abstract

OBJECTIVE: Cigarette smoking is the main risk factor for bladder cancer development. Among the mediators of this effect of smoking is nuclear factor-kappa B. Curcumin suppresses cellular transformation by downregulating the activity of nuclear factor-kappa B. Prima-1 is a compound that induces apoptosis in human tumor cells, restoring the function of mutant p53. Our study aimed to evaluate the effects of curcumin and prima-1 in an animal model of bladder cancer. METHODS: Tumor implantation was achieved in six- to eight-week-old female C57BL/6 mice by introducing MB49 bladder cancer cells into the bladder. Intravesical treatment with curcumin and Prima-1 was performed on days 2, 6, 10, and 14. On day 15, the animals were sacrificed. Immunohistochemistry was used to determine the expression of cyclin D1, Cox-2, and p21. Cell proliferation was examined using PCNA. RESULTS: Animals treated with curcumin exhibited a higher degree of necrosis than animals in other groups. Immunohistochemistry showed reduced expression of cyclin D1 in the curcumin-treated group. All of the cells in mice treated with curcumin were p21 positive, suggesting that the p53 pathway is induced by this compound. Prima-1 did not induce any change in tumor size, necrosis, cell proliferation, or the expression of proteins related to the p53 pathway in this animal model. CONCLUSION: Curcumin showed activity in this animal bladder cancer model and probably acted via the regulation of nuclear factor-kappa B and p53. Therefore, curcumin is a good choice for the use in clinical trials to treat superficial bladder cancer as an alternative to bacillus Calmette-Guerin. In contrast, Prima-1 does not seem to have an effect on bladder cancer.

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Published

2011-01-01

Issue

Section

Basic Researches

How to Cite

Watanabe, F. T., Chade, D. C., Reis, S. T., Piantino, C., Dall’ Oglio, M. F., Srougi, M., & Leite, K. R. M. (2011). Curcumin, but not Prima-1, decreased tumor cell proliferation in the syngeneic murine orthotopic bladder tumor model . Clinics, 66(12), 2121-2124. https://doi.org/10.1590/S1807-59322011001200019