Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
DOI:
https://doi.org/10.1016/j.clinsp.2022.100102Keywords:
COPD, miR-423-5p, GEO, Receiver operating curveAbstract
Objective: This study aimed to examine changes in miRNAs expression profile of COPD patients.
Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression.
Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking.
Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients.
