Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients

Authors

  • Xin Zhang ChongQing TongLiang People's Hospital
  • Qing Shi ChongQing TongLiang People's Hospital
  • Lu Xiong ChongQing TongLiang People's Hospital
  • Shiye Shi ChongQing TongLiang People's Hospital
  • Yong Li ChongQing TongLiang People's Hospital
  • Yanhuan Wang ChongQing TongLiang People's Hospital
  • Mingchuan Zhang ChongQing TongLiang People's Hospital https://orcid.org/0000-0003-1589-2737

DOI:

https://doi.org/10.1016/j.clinsp.2022.100102

Keywords:

COPD, miR-423-5p, GEO, Receiver operating curve

Abstract

Objective: This study aimed to examine changes in miRNAs expression profile of COPD patients.

Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression.

Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking.

Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients.

Downloads

Download data is not yet available.

Downloads

Published

2022-09-23

Issue

Section

Original Articles

How to Cite

Zhang, X., Shi, Q., Xiong, L., Shi, S., Li, Y., Wang, Y., & Zhang, M. (2022). Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients. Clinics, 77, 100102. https://doi.org/10.1016/j.clinsp.2022.100102