Mesenchymal stem cells-derived exosomal miR-653-5p suppresses laryngeal papilloma progression by inhibiting BZW2

Authors

  • Binya Hu Hunan Children's Hospital
  • Min Huang Hunan Children's Hospital
  • Lihua Tao Hunan Children's Hospital
  • Yun Li Hunan Children's Hospital
  • Yuting Kuang Hunan Children's Hospital
  • Guangliang Liu Hunan Children's Hospital
  • Sijun Zhao Hunan Children's Hospital https://orcid.org/0000-0002-3897-9061

DOI:

https://doi.org/10.1016/j.clinsp.2022.100129

Keywords:

Papillomatosis, Laryngeal Diseases, Mesenchymal Stem Cells, MicroRNAs, Exosomes

Abstract

Objectives: Although miR-653-5p has been validated to participate in the progression of multiple types of cancer, the functional role of exosomal miR-653-5p derived from Mesenchymal Stem Cells (MSCs) in Laryngeal Papilloma (LP) has still remained elusive. Hence, this study aimed to investigate the role of MSCs-derived exosomal miR-653-5p in LP.

Methods: LP tissues (n = 15) and adjacent normal tissues (n = 10) were collected to examine the expression level of miR-653-5p. The expression level of miR-653-5p in LP cells and normal cells was also detected. Then, miR-653-5p was overexpressed or silenced to explore its effects on the proliferation, migration, invasion, and apoptosis of LP cells. Thereafter, the effects of exosomal miR-653-5p derived from MSCs on LP cell progression and the potential regulatory mechanism of miR-653-5p were assessed.

Results: It was revealed that the expression level of miR-653-5p was downregulated in LP tissues and cells. In addition, miR-653-5p suppressed the proliferation, migration, invasion, and apoptosis of LP cells. Exosomes derived from MSCs played a suppressive role in LP development and mediated the transmission of miR-653-5p to LP cells. Further exploration identified Basic leucine Zipper and W2 domains 2 (BZW2) as the target of miR-653-5p. More importantly, the rescue experiments revealed that MSCs-secreted exosomal miR-653-5p efficiently inhibited the aggressive phenotypes of LP cells, which could be significantly reversed by BZW2 overexpression in LP cells.

Conclusion: MSCs-derived exosomal miR-653-5p exerted inhibitory effects on LP progression through targeting BZW2, which provided a novel idea for the therapy of LP.

Clinical Trial registration number: chictr-ior-17011021.

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Published

2022-12-05

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Section

Original Articles

How to Cite

Hu, B., Huang, M., Tao, L., Li, Y., Kuang, Y., Liu, G., & Zhao, S. (2022). Mesenchymal stem cells-derived exosomal miR-653-5p suppresses laryngeal papilloma progression by inhibiting BZW2. Clinics, 78, 100129. https://doi.org/10.1016/j.clinsp.2022.100129