The use of vancomycin-loaded poly-l-lactic acid and poly-ethylene oxide microspheres for bone repair: an in vivo study

Authors

  • Débora Cristina Coraca-Huber Medical University Innsbruck; Department of Orthopaedic Surgery; Experimental Orthopaedics
  • Eliana Aparecida de Rezende Duek Campinas State University; Mechanical Engineering Faculty
  • Maurício Etchebehere Campinas State University; Medical Sciences Faculty; Orthopaedic and Traumatology Department
  • Luis Alberto Magna Campinas State University; Medical Sciences Faculty; Medical Genetics
  • Eliane Maria Ingrid Amstalden Campinas State University; Medical Sciences Faculty; Pathological Anatomy Department

DOI:

https://doi.org/10.6061/clinics/2012(07)15

Keywords:

Poly-L-lactic acid, Poly-ethylene oxide, Vancomycin, Bone grafting

Abstract

OBJECTIVE: The aim of this study was to investigate bone repair after the implantation of vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres compared with vancomycin-unloaded poly-L-lactic acid/poly-ethylene oxide microspheres. METHODS: Poly-L-lactic acid/poly-ethylene oxide microspheres were implanted in rat tibiae and evaluated for periods of 2, 4, 8, and 12 days and 4, 8, 16, and 32 weeks. The groups implanted with vancomycin-loaded and vancomycin-unloaded microspheres were compared. Histopathologic (semi-quantitative) and histomorphometric analyses were performed to evaluate the bone formation process. RESULTS: During the first period (second day), fibrin and hemorrhaging areas were observed to be replaced by granulation tissue around the microspheres. Woven bone formation with progressive maturation was observed. All of the histopathological findings, evaluated by a semi-quantitative assay and a quantitative analysis (percentage of bone formation), were similar between the two groups. CONCLUSION: Vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres are a good bone substitute candidate for bone repair. Local antibiotic therapy using vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres should be considered after the microbiological evaluation of its efficacy.

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Published

2012-07-01

Issue

Section

Basic Researches

How to Cite

Coraca-Huber, D. C., Duek, E. A. de R., Etchebehere, M., Magna, L. A., & Amstalden, E. M. I. (2012). The use of vancomycin-loaded poly-l-lactic acid and poly-ethylene oxide microspheres for bone repair: an in vivo study. Clinics, 67(7), 793-798. https://doi.org/10.6061/clinics/2012(07)15