Evaluation of histological changes after tracheal occlusion at different gestational ages in a fetal rat model

Authors

  • Rodrigo Melo Gallindo University of São Paulo; School of Medicine of Ribeirão Preto; Division of Pediatric Surgery; Department of Surgery and Anatomy
  • Frances Lilian Lanhellas Gonçalves University of São Paulo; School of Medicine of Ribeirão Preto; Division of Pediatric Surgery; Department of Surgery and Anatomy
  • Carolina Teixeira de Resende Barreto State University of Campinas (UNICAMP); School of Medical Sciences; Department of Obstetrics and Gynecology
  • Augusto Frederico Santos Schmidt Cincinnati Children's Hospital Medical Center; Department of Pediatrics
  • Luis Antonio Violin Dias Pereira State University of Campinas, UNICAMP; Department of Histology and Embryology
  • Lourenço Sbragia University of São Paulo; School of Medicine of Ribeirão Preto; Division of Pediatric Surgery; Department of Surgery and Anatomy

DOI:

https://doi.org/10.6061/clinics/2013(01)OA09

Keywords:

Therapeutic Occlusion, Trachea, Hernia, Diaphragmatic, Rats, Experimental Model

Abstract

OBJECTIVES: To evaluate the histological changes of tracheal cartilage and epithelium caused by tracheal occlusion at different gestational ages in a fetal rat model. METHODS: Rat fetuses were divided into two groups: a) External control, composed of non-operated rats, and b) Interventional group, composed of rats operated upon on gestational day 18.5 (term = 22 days), divided into triads: 1) Tracheal occlusion, 2) Internal control and 3) Sham (manipulated but not operated). Morphological data for body weight, total lung weight and total lung weight/body weight ratio were collected and measured on gestational days 19.5, 20.5 and 21.5. Tracheal samples were histologically processed, and epithelial, chondral and total tracheal thicknesses were measured on each gestational day. RESULTS: The tracheal occlusion group exhibited an increase in total lung weight/body weight ratio (p<0.001). Histologically, this group had a thicker epithelial thickness (p<0.05) and thinner chondral (p<0.05) and total tracheal thicknesses (p<0.001). These differences were more prominent on gestational days 20.5 and 21.5. CONCLUSION: Tracheal occlusion changed tracheal morphology, increased epithelial thickness and considerably decreased total tracheal thickness. These changes in the tracheal wall could explain the development of tracheomegaly, recently reported in some human fetuses subjected to tracheal occlusion.

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Published

2013-01-01

Issue

Section

Basic Research

How to Cite

Gallindo, R. M., Gonçalves, F. L. L., Barreto, C. T. de R., Schmidt, A. F. S., Pereira, L. A. V. D., & Sbragia, L. (2013). Evaluation of histological changes after tracheal occlusion at different gestational ages in a fetal rat model . Clinics, 68(1), 59-63. https://doi.org/10.6061/clinics/2013(01)OA09