Tamoxifen decreases the myofibroblast count in the healing bile duct tissue of pigs

Authors

  • Orlando Hiroshi Kiono Siqueira Fluminense Federal University; Department of General and Specialized Surgery
  • Benedito Herani Filho São Paulo Federal University; Surgical Gastroenterology
  • Rafael Erthal de Paula Fluminense Federal University; School of Medicine
  • Fábio Otero Áscoli Fluminense Federal University; Biomedical Institute
  • Antonio Cláudio Lucas da Nóbrega Fluminense Federal University; Biomedical Institute
  • Angela Cristina Gouvêa Carvalho Fluminense Federal University
  • Andréa Rodrigues Cordovil Pires Fluminense Federal University
  • Nicolle Cavalcante Gaglionone Fluminense Federal University; Clinical Research Unit
  • Karin Soares Gonçalves Cunha Federal Fluminense University; Postgraduate Program in Pathology
  • José Mauro Granjeiro Federal Fluminense University; Clinical Research Unit

DOI:

https://doi.org/10.6061/clinics/2013(01)OA16

Keywords:

Tamoxifen, Myofibroblasts, Biliary Wound Healing, Bile Duct Stricture, Bile Duct Injury

Abstract

OBJECTIVE: The aim of this study was to evaluate the effect of oral tamoxifen treatment on the number of myofibroblasts present during the healing process after experimental bile duct injury. METHODS: The sample consisted of 16 pigs that were divided into two groups (the control and study groups). Incisions and suturing of the bile ducts were performed in the two groups. Tamoxifen (20 mg/day) was administered only to the study group. The animals were sacrificed after 30 days. Quantification of myofibroblasts in the biliary ducts was made through immunohistochemistry analysis using anti-alpha smooth muscle actin of the smooth muscle antibody. Immunohistochemical quantification was performed using a digital image system. RESULTS: In the animals treated with tamoxifen (20 mg/day), there was a significant reduction in immunostaining for alpha smooth muscle actin compared with the control group (0.1155 vs. 0.2021, p = 0.046). CONCLUSION: Tamoxifen reduced the expression of alpha smooth muscle actin in the healing tissue after bile duct injury, suggesting a decrease in myofibroblasts in the scarred area of the pig biliary tract. These data suggest that tamoxifen could be used in the prevention of biliary tract stenosis after bile duct surgeries.

Downloads

Download data is not yet available.

Downloads

Published

2013-01-01

Issue

Section

Basic Researches

How to Cite

Siqueira, O. H. K., Herani Filho, B., Paula, R. E. de, Áscoli, F. O., Nóbrega, A. C. L. da, Carvalho, A. C. G., Pires, A. R. C., Gaglionone, N. C., Cunha, K. S. G., & Granjeiro, J. M. (2013). Tamoxifen decreases the myofibroblast count in the healing bile duct tissue of pigs . Clinics, 68(1), 101-106. https://doi.org/10.6061/clinics/2013(01)OA16