Bone microdamage evaluation

a supplementary tool to provide three-dimensional visualization

Authors

  • Isabella de Almeida Francisquini Universidade Federal de Juiz de Fora
  • Ricardo Armini Caldas Universidade Federal de Santa Catarina
  • Gustavo Davi Rabelo Universidade Federal de Santa Catarina

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2020.170117

Keywords:

Bone, Microdamage, Microcrack, Fracture, Three-Dimensional Imaging, Computer-Generated 3D Imaging

Abstract

Objectives: This technical report demonstrates the use of a 3D visualization tool to evaluate bone microdamage in histological sections. Methods: Histological sections obtained from non-demineralized bone fragments stained with Calcein and Xylenol Orange and embedded in methyl methacrylate were used. Sections containing microdamages (microcracks, diffuse damage and/or microfractures) were assessed to create a 3D image with the use of polarization and phase-contrast microscopies by light optical and confocal microscopes, respectively. The 3D images were created with the use of a plugin named “Interactive 3D Surface Plot” for ImageJ. Results: Microdamage morphology was better evaluated by means of the 3D image obtained, visualized in both plots of original colors and Spectrum LUT multicolor. The different colors in the Spectrum LUT highlighted the damage and improved visualization. Conclusions: The use of 3D visualization to supplement classic bone microdamage evaluation improves the assessment of damage morphology and enhances their analysis by changing the perspective in different views.

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Author Biographies

  • Isabella de Almeida Francisquini, Universidade Federal de Juiz de Fora

    Faculdade de Odontologia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil

  • Ricardo Armini Caldas, Universidade Federal de Santa Catarina

    Departamento de Odontologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil

  • Gustavo Davi Rabelo, Universidade Federal de Santa Catarina

    Departamento de Odontologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil

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Published

2020-11-16

Issue

2020

Section

Original Research

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Copyright (c) 2020 Isabella de Almeida Francisquini, Ricardo Armini Caldas, Gustavo Davi Rabelo

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