Comparison of different low-level laser therapy wavelengths in the soleus of Wistar rats after nerve injury
DOI:
https://doi.org/10.1590/1809-2950/18019627022020Keywords:
Sciatic Nerve/injuries, Laser Therapy, Physical TherapyAbstract
Skeletal muscles may be affected by peripheral nervous system injuries, leading to muscle weakness and atrophy. Several therapeutic resources may be used in the attempt to recover the functionality of muscles, such as low-level laser therapy (LLLT). This study compared the effect of LLLT of two wavelengths (660 nm and 830 nm) on morphological characteristics of muscle tissue after axonotmesis of ischiatic nerves of Wistar rats. A total of 32 Wistar rats were divided into four groups: G1 (control), G2 (injury), G3 (injury and treatment with 660 nm LLLT) and G4 (injury and treatment with 830 nm LLLT). G2, G3, and G4 animals were submitted to sciatic nerve damage and, three days after the injury, G3 and G4 were treated with LLLT of 660 nm and 830 nm, respectively. After the treatment, all animals were euthanized, and the soles muscles were collected to perform morphological analyzes of the tissue using histological slides. We verified that animals submitted to the lesion underwent morphological changes in the fiber, resulting in their atrophy. We also noticed that LLLT with a wavelength of 830 nm presented slight signs of recovery of the morphometric characteristics analyzed.
Downloads
References
Santana AJ, Debastiani JC, Buratti P, Peretti AL, Kunz RI, Brancalhão
RMC, et al. Sericin and swimming on histomorphometric
parameters of denervated plantar muscle in Wistar rats. Einstein.
;16(1):1-6. doi: 10.1590/s1679-45082018ao4137
Tanaka S, Tsubaki A, Tachino K. Effect of exercise training
after partial denervation in rat soleus muscles. J Phys Ther Sci.
;17(2):97-101. doi: 10.1589/jpts.17.97
Souza MV, Silva MO. Laserterapia em afecções locomotoras: revisão
sistemática de estudos experimentais. Rev Bras Med Esporte.
;22(1):76-82. doi: 10.1590/1517-869220162201144326
Wang C-Z, Chen Y-J, Wang Y-H, Yeh M-L, Huang M-H, Ho M-L,
et al. Low-level laser irradiation improves functional recovery
and nerve regeneration in sciatic nerve crush rat injury model.
PLoS One. 2014;9(8):e103348. doi: 10.1371/journal.pone.0103348.
Buchaim RL, Andreo JC, Barraviera B, Ferreira Junior RS,
Buchaim DV, Rosa Júnior GM, et al. Effect of low-level laser
therapy (LLLT) on peripheral nerve regeneration using fibrin
glue derived from snake venom. Injury. 2015;46(4):655-60.
doi: 10.1016/j.injury.2015.01.031.
Andreo L, Soldera CB, Ribeiro BG, Matos PRV, Bussadori
SK, Fernandes KPS, et al. Effects of photobiomodulation on
experimental models of peripheral nerve injury. Lasers Med
Sci. 2017;32(9):2155-65. doi: 10.1007/s10103-017-2359-7.
Gonçalves RB, Marques JC, Monte-Raso VV, Zamarioli A, Carvalho
LC, Fazan VPS, et al. Efeitos da aplicação do laser de baixa
potência na regeneração do nervo isquiático de ratos. Fisioter
Pesqui. 2010;17(1):34-9. doi: 10.1590/S1809-29502010000100007.
Takhtfooladi MA, Jahanbakhsh F, Takhtfooladi HA, Yousefi
K, Allahverdi A. Effect of low-level laser therapy (685 nm,
J/cm2
) on functional recovery of the sciatic nerve in rats
following crushing lesion. Lasers Med Sci. 2015;30(3):1047-52.
doi: 10.1007/s10103-015-1709-6.
Barbosa RI, Marcolino AM, Guirro RRJ, Mazzer N, Barbieri CH,
Fonseca MCR. Efeito do laser de baixa intensidade (660 nm) na
regeneração do nervo isquiático lesado em ratos. Fisioter Pesqui.
;17(3):294-9. doi: 10.1590/S1809-29502010000400002.
Rosa Júnior GM, Magalhães RMG, Rosa VC, Bueno CRS,
Simionato LH, Bortoluci CHF. Effect of the laser therapy
in association with swimming for a morphological nerve
repair and functional recovery in rats submitted to
sciatic axonotmesis. Fisioter Pesqui. 2016;23(1):12-20.
doi: 10.1590/1809-2950/13929623012016.
Bagis S, Comelekoglu U, Coskun B, Milcan A, Buyukakilli B,
Sahin G, et al. No effect of GA-AS (904 nm) laser irradiation
on the intact skin of the injured rat sciatic nerve. Lasers Med
Sci. 2003;18(2):83-8. doi: 10.1007/s10103-003-0258-6.
Oliveira RF, Andrade Salgado DMR, Trevelin LT, Lopes RM,
Cunha SRB, Aranha ACC, et al. Benefits of laser phototherapy
on nerve repair. Lasers Med Sci. 2015;30(4):1395-406.
doi: 10.1007/s10103-014-1531-6.
Smith KC. Molecular targets for low-level light therapy. Laser
Ther. 2010;19(3):135-42. doi: 10.5978/islsm.19.135.
Bridge PM, Ball DJ, Mackinnon SE, Nakao Y, Brandt K, Hunter
DA, et al. Nerve crush injuries: A model for axonotmesis. Exp
Neurol. 1994;127(2):284-90. doi: 10.1006/exnr.1994.1104.
Santana AJ, Debastiani JC, Kunz RI, Buratti P, Brancalhão RMC,
Ribeiro LFC, et al. Association of sericin and swimming on the
phenotype, motor plate, and functionality of the denervated
plantar muscle of Wistar rats. J Exerc Rehabil. 2018;14(1):24-31.
doi: 10.12965/jer.1835138.569.
Machado M. Atualidades em fisiologia do músculo
esquelético: célula satélite e hipertrofia. Rev Perspect Online.
;5(1):116-20.
Lovison K, Vieira L, Kunz RI, Scarton SRS, Antunes S, Karvat
J, et al. Resistance exercise recovery morphology and AQP1
expression in denervated soleus muscle of Wistar rats.
Motricidade. 2018;14(1):40-50. doi: 10.6063/motricidade.11788.
Stein T, Souza-Silva E, Mascarin L, Eto C, Fin FE, Tonussi CR.
Histaminergic pharmacology modulates the analgesic and
antiedematogenic effects of spinally injected morphine. Anesth
Analg. 2016;123(1):238-43. doi: 10.1213/ANE.0000000000001326.
Downloads
Published
Issue
Section
License
Copyright (c) 2020 Daniele Pelissari, Lucinéia de Fátima Chasko Ribeiro, Luiz Gustavo Vasconcelos Machado, Morgana Neves, Rose Meire Costa, Gladson Ricardo Flor Bertolini
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
