Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins

Authors

  • Daniele Lucca LONGO Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil
  • Francisco Wanderley Garcia PAULA-SILVA Universidade de São Paulo; Faculdade de Ciências Farmacêuticas de Ribeirão Preto; Departamento de Análises Clínicas
  • Lucia Helena FACCIOLI Universidade de São Paulo; Faculdade de Ciências Farmacêuticas de Ribeirão Preto; Departamento de Análises Clínicas
  • Patrícia Maria GATÓN-HERNÁNDEZ Universitat de Barcelona; Facultat d’Odontologia; Department d’Odontopediatria, Barcelona
  • Alexandra Mussolino de QUEIROZ Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil
  • Léa Assed Bezerra da SILVA Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil

DOI:

https://doi.org/10.1590/1678-775720150449

Abstract

The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05). Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>;0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.

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Published

2016-08-01

Issue

Section

Original Articles

How to Cite

LONGO, D. L., PAULA-SILVA, F. W. G., FACCIOLI, L. H., GATÓN-HERNÁNDEZ, P. M., QUEIROZ, A. M. de, & SILVA, L. A. B. da. (2016). Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins . Journal of Applied Oral Science, 24(4), 338-343. https://doi.org/10.1590/1678-775720150449