Longitudinal bond strength of a universal adhesive and chemical dentin characterization under different acid etching protocols

Lucélia Lemes Gonçalves; Anuradha Prakki; Tânia Mara da Silva; Arwa Bafail; Janaína Bortolatto; Alexander Terry Stavroullakis; Sérgio Eduardo de Paiva Gonçalves

doi:10.1590/1678-7757-2023-0359

Authors

Lucélia Lemes Gonçalves Universidade Estadual Paulista (UNESP), Instituto de Ciência e Tecnologia da UNESP, Departamento de Odontologia Restauradora, São José dos Campos https://orcid.org/0000-0002-1973-5854
Anuradha Prakki University of Toronto, Faculty of Dentistry, Dental Research Institute, Toronto, ON https://orcid.org/0000-0002-8969-9043
Tânia Mara da Silva Universidade Estadual Paulista (UNESP), Instituto de Ciência e Tecnologia da UNESP, Departamento de Odontologia Restauradora, São José dos Campos https://orcid.org/0000-0001-6344-6905
Arwa Bafail Taibah University, College of Dentistry, Department of Restorative Dental Sciences, Madinah https://orcid.org/0000-0002-3687-4207
Janaína Bortolatto University of Toronto, Faculty of Dentistry, Dental Research Institute, Toronto, ON https://orcid.org/0000-0002-6584-7136
Alexander Terry Stavroullakis University of Toronto, Faculty of Dentistry, Dental Research Institute, Toronto, ON https://orcid.org/0000-0002-8566-787X
Sérgio Eduardo de Paiva Gonçalves Universidade Estadual Paulista (UNESP), Instituto de Ciência e Tecnologia da UNESP, Departamento de Odontologia Restauradora, São José dos Campos https://orcid.org/0000-0003-1796-0393

DOI:

https://doi.org/10.1590/1678-7757-2023-0359

Keywords:

Dentin, Acid etching, Adhesives, Matrix metalloproteinases, Collagen

Abstract

This study aimed to analyze the longitudinal bond strength of a universal adhesive and chemically characterize the dentin substrate under different acid etching protocols. Methodology: Dentin samples were etched with polyacrylic acid 25% (PAA) for 10 seconds (n=3) and phosphoric acid 32% (PA) for 15 seconds (n=3) and analyzed by Fourier transform infrared spectroscopy – attenuated total reflectance (FTIR-ATR) before and after treatment. For collagen degradation, samples (n=12) were divided into 3 groups: PAA, PA, and Deionized water (control), and analyzed by the quantity of solubilized type I collagen C-terminal cross-linked telopeptides and solubilized C-terminal peptide in relation to total protein concentration (ICTPtp and CTXtp) and by their ultimate tensile strength (UTS). For the adhesive interface analysis, dentin samples (n=72) were divided into 3 groups: PAA, PA, and Self-etch (SE), and subdivided into 2 groups: 24 h (baseline) and 1 year. The following tests were performed: microtensile bond strength (μTBS) (n=48), scanning electron microscopy (SEM) (n=12), and nanoleakage (n=12). Results: The FTIR of PAA showed lower reduction of the peaks in the phosphate group when compared to PA. For ICTPtp, PA showed a significantly higher value. For CTXtp, PA and PAA groups failed to statically differ from each other. UTS was significantly lower for PA. For μTBS, storage time significantly affected bond strength. The results were unaffected by the etching protocol. For SEM, after 1 year, PA had little evidence of degradation in the upper third of the adhesive interface in comparison to the other groups. Nanoleakage showed no considerable silver impregnation after 1 year in the SE group. Conclusion: The use of PAA prior to a universal adhesive (when compared to PA) represents a less aggressive type of etching to dentin. However, self-etching still seems to be the best option for universal adhesive systems that have functional monomers in their composition.

Downloads

Download data is not yet available.

References

Van Meerbeek B, Yoshihara K, Van Landuyt K, Yoshida Y, Peumans M. From Buonocore's pioneering acid-etch technique to self-adhering restoratives. A status perspective of rapidly advancing dental adhesive technology. J Adhes Dent. 2020;22(1):7-34. doi: 10.3290/j.jad.a43994

Tjäderhane L. Dentin bonding: can we make it last? Oper Dent. 2015;40:4-18. doi: 10.2341/14-095-BL

Breschi L, Maravic T, Cunha SR, Comba A, Cadenaro M, Tjäderhane L, et al. Dentin bonding systems: from dentin collagen structure to bond preservation and clinical applications. Dent Mater. 2018;34(1):78-96. doi: 10.1016/j.dental.2017.11.005

Jin X, Han F, Wang Q, Yuan X, Zhou Q, Xie H, et al. The roles of 10-methacryloyloxydecyl dihydrogen phosphate and its calcium salt in preserving the adhesive–dentin hybrid layer. Dent Mater. 2022;38(7):1194-205. doi: 10.1016/j.dental.2022.06.007

Silva TM, Petrucelli NF, Mendonça RP, Silva RP Jr, Campos, TM, Gonçalves SE. Impact of photoinitiator quality on chemical-mechanical properties of dental adhesives under different light intensities. Braz Dent Sci. 2023;26(spec ed.): doi: 10.4322/BDS.2023.E3704

Perdigão J. Current perspectives on dental adhesion: (1) Dentin adhesion – not there yet. Jpn Dent Sci Rev. 2020;56(1):19222207. doi: 10.1016/j.jdsr.2020.08.004

Sulkala M, Tervahartiala T, Sorsa T, Larmas M, Salo T, Tjäderhane L. Matrix metalloproteinase-8 (MMP-8) is the major collagenase in human dentin. Arch Oral Biol. 2007;52(2):121-7. doi: 10.1016/j.archoralbio.2006.08.009

Ozcan S, Seseogullari-Dirihan R, Uctasli M, Tay FR, Pashley DH, Tezvergil-Mutluay A. Effect of polyacrylic acid on dentin protease activities. Dent Mater. 2015;31(8):901-6. doi: 10.1016/j.dental.2015.04.018

Tezvergil-Mutluay A, Mutluay M, Seseogullari-Dirihan R, Agee KA, Key WO, Scheffel DL, et al. Effect of phosphoric acid on the degradation of human dentin matrix. J Dent Res. 2013;92(1):87-91. doi: 10.1177/0022034512466264

Loguercio AD, Paula EA, Hass V, Luque-Martinez I, Reis A, Perdigão J. A new universal simplified adhesive: 36-Month randomized double-blind clinical trial. J Dent. 2015;43(9):1083-1092. doi: 10.1016/j.jdent.2015.07.005

Saikaew P, Sattabanasuk V, Harnirattisai C, Chowdhury AF, Carvalho R, Sano H. Role of the smear layer in adhesive dentistry and the clinical applications to improve bonding performance. Jpn Dent Sci Rev. 2022;58:59-66. doi: 10.1016/j.jdsr.2021.12.001

Pavan S, Santos PH, Berger S, Bedran-Russo AK. The effect of dentin pretreatment on the microtensile bond strength of self-adhesiveresin cements. J Prosthet Dent. 2010;104(4):258-64. doi: 10.1016/S0022-3913(10)60134-5

Botta SB, Ana PA, Santos MO, Zezell DM, Matos AB. Effect of dental tissue conditioners and matrix metalloproteinase inhibitors on type I collagen microstructure analyzed by Fourier transform infrared spectroscopy. J Biomed Mater Res B Appl Biomater. 2012; 100(4):1009-16. doi: 10.1002/jbm.b.32666

Maske TT, Kuper NK, Hollanders AC, Bronkhorst EM, Cenci MS, Huysmans MC. Secondary caries development and the role of a matrix metalloproteinase inhibitor: a clinical in situ study. J Dent. 2018;71:49-53. doi: 10.1016/j.jdent.2018.01.011

Sylvester MF, Yannas IV, Salzman EW, Forbes MJ. Collagen banded fibril structure and the collagen-platelet reaction. Thromb Res. 19891;55(1):135-48. doi: 10.1016/0049-3848(89)90463-5

Bafail A, Carrilho MR, Kishen A, Prakki A. Effect of protease inhibitor specificity on dentin matrix properties. J Mech Behav Biomed Mater. 2020;109:103861. doi: 10.1016/j.jmbbm.2020.103861

Armstrong S, Breschi L, Özcan M, Pfefferkorn F, Ferrari M, Van Meerbeek B. Academy of Dental Materials guidance on in vitro testing of dental composite bonding effectiveness to dentin/enamel using micro-tensile bond strength (μTBS) approach. Dent Mater. 2017;33(2):133-43. doi: 10.1016/j.dental.2016.11.015

Gonçalves LL, Silva TM, Prakki A, Barcellos DC, Caneppele TM, De Oliveira HP, et al. Universal adhesive: the effect of different simulated pulpal pressure fluids and bonding modes to dentin. Odontology. 2022;110(1):62-9. doi: 10.1007/s10266-021-00633-0

Tay FR, Pashley DH, Yoshiyama M. 2 modes of nanoleakage expression in single-step adhesives. J Dent Res. 2002;81(7):472-6. doi: 10.1177/154405910208100708

Souza MY, Andrade JL, Ferraz Caneppele TM, et al. Assessment of nanohardness, elastic modulus, and nanoleakage of the adhesive interface using the ethanol-wet-bonding technique. Int J Adhes Adhes 2020;99:102572. doi: 10.1016/j.ijadhadh.2020.102572

Yang B, Adelung R, Ludwig K, Bössmann K, Pashley DH, Kern M. Effect of structural change of collagen fibrils on the durability of dentin bonding. Biomaterials. 2005;26(24):5021-31. doi: 10.1016/j.biomaterials.2005.01.024

Amin F, Fareed MA, Zafar MS, Khurshid Z, Palma PJ, Kumar N. Degradation and stabilization of resin-dentine interfaces in polymeric dental adhesives: an updated review. Coatings. 2022;12(8):1094. doi: 10.3390/coatings12081094

Wendlinger M, Pomacóndor-Hernández C, Pintado-Palomino K, Cochinski GD, Loguercio AD. Are universal adhesives in etch-and-rinse mode better than old 2-step etch-and-rinse adhesives? 1-year evaluation of bonding properties to dentin. J Dent. 2023;132:104481. doi: 10.1016/j.jdent.2023.104481

Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, et al. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004;83(3):216-21. doi: 10.1177/154405910408300306

Hu L, Xiao YH, Fang M, Gao Y, Huang L, Jia AQ, et al. Effects of type I collagen degradation on the durability of 3 adhesive systems in the early phase of dentin bonding. PLoS 1. 2015;10(2):e0116790. doi: 10.1371/journal.pone.0116790

DeVito-Moraes AG, Francci C, Vidal CM, Scaffa PM, Nesadal D, Yamasaki LC, et al. Phosphoric acid concentration affects dentinal MMPs activity. J Dent. 2016;53:37777. doi: 10.1016/j.jdent.2016.06.002

Tersariol IL, Geraldeli S, Minciotti CL, Nascimento FD, Pääkkönen V, Martins MT, et al. Cysteine cathepsins in human dentin-pulp complex. J Endod. 2010;36(3):475-81. doi: 10.1016/j.joen.2009.12.034

Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol IL, Geraldeli S, et al. Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins. Dent Mater. 2013;29(1):116-35. doi: 10.1016/j.dental.2012.08.004

Sai K, Shimamura Y, Takamizawa T, Tsujimoto A, Imai A, Endo H, et al. Influence of degradation conditions on dentin bonding durability of 3 universal adhesives. J Dent. 2016;54:56-61. doi: 10.1016/j.jdent.2016.09.004

Marchesi G, Frassetto A, Mazzoni A, Apolonio F, Diolosà M, Cadenaro M, et al. Adhesive performance of a multi-mode adhesive system: 1-year in vitro study. J Dent. 2014;42(5):603-12. doi: 10.1016/j.jdent.2013.12.008

Sano H. Microtensile testing, nanoleakage, and biodegradation of resin-dentin bonds. J Dent Res. 2006;85(1):11-4. doi: 10.1177/154405910608500102

El Gezawi M, Haridy R, Abo Elazm E, Al-Harbi F, Zouch M, Kaisarly D. Microtensile bond strength, 4-point bending and nanoleakage of resin-dentin interfaces: effects of 2 matrix metalloproteinase inhibitors. J Mech Behav Biomed Mater. 2018;78:206-213. doi: 10.1016/j.jmbbm.2017.11.024

Kinder GR, Furuse AY, Costa RM, Lucena F, Correr G, Gonzaga C. Effect of MDP concentration and addition of iodonium salt on the dentin bond strength of experimental adhesives. Braz Dent Sci. 2022;25(1):e2933. doi: 10.4322/BDS.2022.E2933

Xu X, Chen Q, Lederer A, Bernau C, Lai G, Kaisarly D, et al. Shear bond strength of 2 adhesives to bovine dentin contaminated with various astringents. Am J Dent. 2015;28(4):229-34.

Longitudinal bond strength of a universal adhesive and chemical dentin characterization under different acid etching protocols

Authors

DOI:

Keywords:

Abstract

Downloads

References

Downloads

Published

Issue

Section

License

How to Cite

Language

Information

Keywords