n vitro effect of TiF4/NaF solution on the development of radiation-induced dentin caries

Abstract

Objective: To evaluate the protective effect of an experimental solution containing TiF₄/NaF on the development of radiation-induced dentin caries lesions. Methodology: bovine root samples were irradiated (70Gy) and distributed as following (n=12/group): Commercial Saliva (BioXtra), NaF (500 ppm F-), TiF₄ (500 ppm F), TiF₄/NaF (TiF₄: 300 ppm F-, NaF: 190 ppm F-), and Phosphate buffer solution (PBS, negative control). Biofilm was produced using biofilm from irradiated patients and McBain saliva (0.2% of sucrose, at 37oC and 5% CO₂) for five days. The treatments were applied 1x/day. Colony-forming units (CFU) were counted and demineralization was quantified by transversal microradiography. The ANOVA/Tukey test was applied for all parameters. Results: All treatments reduced CFU for total microorganisms. TiF₄ reduced Lactobacillus sp. (7.04±0.26 log10 CFU/mL) and mutans streptococci (7.18±0.28) CFU the most, when compared to PBS (7.58±0.21 and 7.75±0.17) and followed by NaF (7.12±0.31 and 7.34±0.22) and TiF₄/NaF (7.16±0.35 and 7.29± 0.29). TiF₄ and Commercial saliva showed the lowest integrated mineral loss (ΔZ-vol%.mm) (1977±150 and 2062±243, respectively) when compared to PBS (4540±335), followed by NaF (2403±235) and TiF₄/NaF (2340±200). Commercial saliva was the only to significantly reduce mineral loss (LD-µm) (111±25) compared to PBS (153±24).Mean mineral loss (R-vol%) decreased by 35.2% for TiF₄ (18.2±3.3) when compared to PBS (28.1±2.9) Conclusion: TiF₄/NaF has a comparable anti-cariogenic effect to TiF₄ and Commercial saliva under the model in this study.