Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats

Authors

  • Samira Esteves Afonso Camargo São Paulo State University; São José dos Campos Dental School; Department of Bioscience and Oral Diagnosis
  • Sigmar de Mello Rode São Paulo State University; São José dos Campos Dental School; Department of Dental Materials and Prosthodontics
  • Renata Falchete do Prado Superior School of Cruzeiro; Department of Physiotherapy
  • Yasmin Rodarte Carvalho São Paulo State University; São José dos Campos Dental School; Department of Bioscience and Oral Diagnosis
  • Carlos Henrique Ribeiro Camargo São Paulo State University; São José dos Campos Dental School; Department of Restorative Dentistry

DOI:

https://doi.org/10.1590/S1678-77572010000300014

Keywords:

Calcium hydroxide, Castor oil, Pulp capping, Rats

Abstract

Castor oil bean cement (COB) is a new material that has been used as an endodontic sealer, and is a candidate material for direct pulp capping. OBJECTIVE: The purpose of this study was to evaluate the biocompatibility of a new formulation of COB compared to calcium hydroxide cement (CH) and a control group without any material, in the subcutaneous tissue of rats. MATERIAL AND METHODS: The materials were prepared, packed into polyethylene tubes, and implanted in the rat dorsal subcutaneous tissue. Animals were sacrificed at the 7th and 50th days after implantation. A quantitative analysis of inflammatory cells was performed and data were subjected to ANOVA and Tukey's tests at 5% significance level. RESULTS: Comparing the mean number of inflammatory cells between the two experimental groups (COB and CH) and the control group, statistically significant difference (p=0.0001) was observed at 7 and 50 days. There were no significant differences (p=0.111) between tissue reaction to CH (382 inflammatory cells) and COB (330 inflammatory cells) after 7 days. After 50 days, significantly more inflammatory cells (p=0.02) were observed in the CH group (404 inflammatory cells) than in the COB group (177 inflammatory cells). CONCLUSIONS: These results demonstrate that the COB cement induces less inflammatory response within long periods.

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Published

2010-06-01

Issue

Section

Original Articles

How to Cite

Camargo, S. E. A., Rode, S. de M., Prado, R. F. do, Carvalho, Y. R., & Camargo, C. H. R. (2010). Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats . Journal of Applied Oral Science, 18(3), 273-278. https://doi.org/10.1590/S1678-77572010000300014