Biofilm disclosing agents in complete denture: clinical and antimicrobial evaluation

Abstract

This study evaluated the disclosing ability, removal facility and antimicrobial effect of biofilm disclosing agents applied on complete dentures. Disclosing ability was evaluated by means of the visual method. The solutions were applied on the internal surface of dentures. After being photographed, the dentures were brushed with denture-specific brush and dentifrice and photographed again. The obtained slides were projected on paper (10 X amplification) and the total and stained surfaces were outlined with graphite, cut off and weighed, in order to assess removal facility. The evaluation of antimicrobial effects was carried out by means of the method of diffusion in agar, and the results were obtained by measuring the length of the halos and rings. In terms of disclosing ability, the best solutions were 0.05% methylene blue, 5% erythrosin, 1% sodic fluorescein, Replak and 1% neutral red. One percent eosin, 1% sodic fluorescein and 5% erythrosin were the most easily removed solutions. One percent eosin, 5% erythrosin, 1% sodic fluorescein, 0.3% proflavine, Replak and 1% neutral red presented no antimicrobial effect. The solutions which presented the greatest disclosing ability and removal facility as well as absence of antimicrobial effect - which are essential requirements in the assessment of hygiene methods and guidance on oral health - were 1% eosin, 1% neutral red and 5% erythrosin.