Validation of analytical methods for the determination of active substances in pharmaceutical preparations used in chemical peelings

Authors

  • Tatiane Rodrigues Ramos Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Farmácia
  • Maria Inês Rocha Miritello Santoro Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Farmácia
  • Erika Rosa Maria Kedor-Hackmann Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Farmácia
  • Anil Kumar Singh Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Farmácia

DOI:

https://doi.org/10.1590/S1516-93322005000200011

Keywords:

Chemical peelings, Salicylic acid, Resorcinol, Derivative spectrophotometry

Abstract

Chemical peeling is obtained with exfoliating formulations and is used in the treatment of actinic keratosis, wrinkles, dyschromies, acne vulgaris and rosacea acne. In this research we selected the Jessner Solution (JS), a pharmaceutical preparation composed of resorcinol (RS) (14%), salicylic acid (SA) (14%) and lactic acid (LA) (14%) in alcoholic solution and two gel samples composed of RS (30%) and SA (20%), respectively. First and second derivative UV spectrophotometric methods were developed and validated for determination of SA and RS, respectively in JS alcoholic solution, ethanol was used as background. A first derivative UV spectrophotometric method was developed for determination of these active substances in gel samples using 0.1N sulfuric acid as background. For SA in the JS, the correlation coefficient (r) was 0.9999, the precision expressed by the relative standard deviation (RSD) of 0.68% and the accuracy expressed by the average percent recovery of 100.5%. For RS in the JS, the r was 0.9999, the RSD of 0.83% and average recovery of 100.3%. In the gel of SA r was 0.9999, the mean RSD was 0.28% and average recovery of 99.3%. In the gel of RS the r was 0.9998, mean RSD was 0.34% and average recovery of 99.9%.

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Published

2005-06-01

Issue

Section

Original Papers

How to Cite

Validation of analytical methods for the determination of active substances in pharmaceutical preparations used in chemical peelings. (2005). Revista Brasileira De Ciências Farmacêuticas, 41(2), 229-235. https://doi.org/10.1590/S1516-93322005000200011