Diagnostic of rifampicin-resistance of Mycobacterium tuberculosis by polymerase chain reaction
DOI:
https://doi.org/10.1590/S1516-93322007000100004Keywords:
Mycobacterium tuberculosis, Resistance, Rifampicin, PCRAbstract
The resistance of Mycobacterium tuberculosis to tuberculostatic drugs has emerged as a major public health threat. The resistance to rifampicin which has been attributed to structural changes in RNA polymerase can be considered as a marker for multi-drug-resistance to tuberculosis (MDR-TB). Patients bearing rifampicin-resistant strains have poor diagnosis even with treatment. Conventional culture-based drug sensibility testing can require several weeks due to the growth. In this paper we describe the most common PCR-based methods for detection of mutations that lead to rifampicin resistance, such as Single-Strand Conformation Polymorphism (SSCP), PCR Heteroduplex and INNO-LIPA. Recently, by Low Stringency using a Single Specific Primer (LSSP) assay, it was standardized a protocol that showed to be rapid and sensitive for the detection of mutations in the rpoB gene.Downloads
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Published
2007-03-01
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Current Comment Article
How to Cite
Diagnostic of rifampicin-resistance of Mycobacterium tuberculosis by polymerase chain reaction. (2007). Revista Brasileira De Ciências Farmacêuticas, 43(1), 31-38. https://doi.org/10.1590/S1516-93322007000100004