Preanalytical and validation studies for the determination of malondialdehyde in human plasma through high performance liquid chromatography after derivatization with 2,4-dinitrophenylhydrazine
DOI:
https://doi.org/10.1590/S1516-93322008000200013Keywords:
Oxidative stress, Malondialdehyde, High performance liquid chromatography, 2, 4-dinitrophenylhydrazineAbstract
Malondialdehyde (MDA) is an important biomarker for the evaluation of oxidative stress status. The aim of this study was to develop a method for plasma MDA quantification by high performance liquid chromatography with diode-array detection (HPLC-DAD), following derivatization with 2,4-dinitrophenylhydrazine (DNPH), evaluating the main preanalytical variables. The calibration curve in plasma (0 to 40 µM) presented high linearity (r² = 0.998). Main validation parameters were: recovery: 78%; LOD: 0.11 µM and LOQ: 0.38 µM. The MDA values obtained in healthy volunteers (n=38) were 3.31 ± 0.38 µM (mean ± SD). Stability studies of MDA standard solution and derivatizing reagent and plasma MDA indicated that the standard solution can be stored at -20 and 4 ºC, remaining stable for at least 30 days.Downloads
Download data is not yet available.
Downloads
Published
2008-06-01
Issue
Section
Original Papers
How to Cite
Preanalytical and validation studies for the determination of malondialdehyde in human plasma through high performance liquid chromatography after derivatization with 2,4-dinitrophenylhydrazine. (2008). Revista Brasileira De Ciências Farmacêuticas, 44(2), 279-287. https://doi.org/10.1590/S1516-93322008000200013