Validation of the i-STAT® CG8+ cartridge analyzer for blood gas analysis
DOI:
https://doi.org/10.11606/issn.1981-4690.2024e38188736Keywords:
Validation studies, Physical activity, Blood gas analysis, Acid base balanceAbstract
The aim of this study was to compare values arterial blood gas values obtained by laboratory technical reference with values obtained by the i-STAT® CG8+ analyzer, in order to confirm their validity and thus extrapolate its applicability in academic research in the context of physical activity. Were collected 22 samples of blood from the radial artery of the non-dominant arm of patients admitted to the ITU of the Hospital São João Batista, located in Viçosa, Minas Gerais, in the period from February to March 2013. Were compared between the reference method Ciba Corning Gas System 278 and i-STAT® CG8+ portable analyzer the result of the following variables: pH; PO2 and PCO2 expressed in mmHg; HCO3, and TCO2 BE (base excess) expressed in mmol / L; sO2 and expressed as a percentage of saturation. The student t test, correlation test, and Bland-Altman analysis were used. For all blood parameters monitored in the comparison of mean values between the two methods, no significant difference was found for any of the variables. There was a significant correlation (p<0.0001) and strong, with all correlation coefficients (r) above 0.93, between the two methods for all variables. The Bland-Altman analysis showed that there was agreement between the two methods, with over 90% of the sample between the limits of agreement for all variables. The i-STAT® CG8+ analyzer presented acceptable validity in the resting situation by reference to the Ciba Corning 278 Gas System device, and its use is an excellent strategy for research in the area of sport and health sciences that require fast results in the resting situation and that ensure the effectiveness of decision making.
Downloads
References
Lindinger MI. Exercise: a paradigma for multi-system control of acid-base state. J Physiol. 2003;550(2):334.
Feriel J, Tchipeva D, Depasse F. Effects of circadian variation, lifestyle and environment on hematological parameters: a narrative review. Int J Lab Hematol. 2021;43:917-926.
Stopyra JP, Snavely AC, Scheidler JF, et al. Point-of-care troponin testing during ambulance transport to detect acute myocardial infarction. Prehosp Emerg Care. 2020;24(6):751-759.
Zaninotto M, Mion MM, Padoan A, et al. Cardiac troponin I in SARS-CoV-2-patients: the additional prognostic value of serial monitoring. Clin Chim Acta. 2020;511:75-80.
Kishbaugh JC, Valitutto MT, Aung O, et al. Use of a portable analyzer for venous blood gas and biochemistry analysis in free-ranging Indian flying foxes (Pteropus giganteus) in Myanmar. J Wildl Dis. 2021;57(1):242-245.
Smith JS, Varga A, Schober KE. Comparison of two commercially available immunoassays for the measurement of bovine cardiac troponin I in Cattle with induced myocardial injury. Front Vet Sci. 2020;27(7):531.
Kipps C, Sharma C, Pedoe DD. The incidence of exercise-associated hyponatraemia in the London marathon. Br J Sports Med. 2011;45:14-19.
Bonaventura JM, Sharp K, Knight E, et al. Reliability and accuracy of six hand-held blood lactate analysers. J Sports Sci Med. 2015;14:203-214.
Mock T, Morrison D, Yatscoff R. Evaluation of the i-STAT ® System: a portable chemistry analyzer for the measurement of Sodium, Potassium, Chloride, Urea, Glucose, and Hematocrit. Clin Biochem. 1995;28:187-192.
Gault MH, Harding CE. Evaluation of i-STAT ® portable clinical analyzer in a hemodialysis unit. Clin Biochem. 1996;29:117-124.
Verwaerde P, Malet C, Lagente M, et al. The accuracy of the i-STAT ® portable analyser for measuring blood gases and pH in whole-blood samples from dogs. Res Vet Sci. 2002;73:71-75.
Dascombe BJ, Reaburn PRJ, Sirotic AC, et al. The reliability of the i-STAT ® clinical portable analyser. J Sci Med Sport. 2007;10:135-140.
Block DR, Wockenfus AM, Koch CD, et al. Analytical performance of three point of care methods for pleural fluid pH analysis. Clin Biochem. 2013;46:1139-1141.
Płoszczyca K, Czuba M, Chalimoniuk M, et al. Red blood cell 2,3-Diphosphoglycerate decreases in response to a 30 km time trial under hypoxia in cyclists. Front Physiol. 2021;15(12):670977.
Newbury JW, Cole M, Kelly AL, et al. The time to peak blood bicarbonate (HCO3-), pH, and the strong ion difference (SID) following sodium bicarbonate (NaHCO3) ingestion in highly trained adolescent swimmers. PLoS One. 2021;16(7):e0248456.
Wiecek M, Maciejczyk M, Szymura J, et al. Changes in oxidative stress and acid-base balance in men and women following maximal-intensity physical exercise. Physiol Res. 2015;64:93-102.
Petrício AIM, Porto M, Burini RC. Alterações hemodinâmicas, do equilíbrio ácido básico e enzimáticas no exercício exaustivo com pesos. Rev Bras Ativ Fís Saúde. 2001;6:17-26.
Trulock EP. Arterial blood gases. In: Walker HK, Hall WD, Hurst JW. Clinical Methods: the history, physical, and laboratory examinations. 3. ed. Boston: Butterworths; 1990. p. 254-257.
Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986; i:307-310.
Garcia LMT, Osti RFI, Ribeiro EHC, et al. Validação de dois questionários para a avaliação da atividade física em adultos. Rev Bras Ativ Fís Saúde. 2013;18:317-331.
Pessoa IMBS, Pereira HLA, Aguiar LT, et al. Reprodutibilidade teste-reteste e validade concorrente de manovacuômetro digital. Fisioter Pesq. 2014;21:236-242.
Hirakata VN, Camey SA. Análise de concordância entre métodos de Bland-Altman. Rev HCPA. 2009;29:261-268.
Bingham D, Kendall J, Clancy M. The portable laboratory: an evaluation of the accuracy and reproducibility of i-STAT®. Ann Clin Biochem. 1999;36:66-71.
Scott MG, LeGrys VA, Klutts JS. Electrolytes and blood gases. In: Burtis CA, Ashwood ER, Bruns DE, organizers. Fundamentals of clinical chemistry and molecular diagnostics (Tietz). Philadelphia: Saunders Elsevier; 2014. p. 412-429.
Oliveira JR, Lunardelli A, Meller KLG, Pereira LLM. Equilíbrio acidobásico. In: Oliveira J, Wachter PH, Azambuja AA, organizadores. Biofísica: para ciências biomédicas. Rio Grande do Sul: EDIPUCRS; 2014. p. 241-268.
Faria VC, Marins JCB, Oliveira GA, et al. Metabolic response to different glycemic indexes of pre-exercise meal. Rev Bras Med Esporte. 2015;21:287-291.
Silva JAJ, Souza MVC, Tomaz LM, et al. Comportamento da lactacidemia e equilíbrio ácido-básico através de técnica pouco invasiva após exercício resistido. RBPFEX. 2019;13(81):43-50.
Faria VC, Marins JCB, Sales SS, et al. Venous blood gases and cardiorespiratory parameters during aerobic exercise with different pre-exercise diet and hydration. Science & Sports. 2016;31:347-354.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Brazilian journal of physical education and sport
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Todo o conteúdo da revista, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (CC-BY)
