Analysis of genetic variability by RAPD of strains isolated from soil and sludge impacted with industrial effluent

Authors

  • Ramon Gomes da Silva Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Departamento de Bioquímica
  • Fernanda Romanholi Pinhati Universidade do Estado do Rio de Janeiro. Faculdade de Tecnologia. Departamento de Química e Ambiental
  • Joab Trajano Silva Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Departamento de Bioquímica

DOI:

https://doi.org/10.7594/revbio.14.01.01

Keywords:

Sludge and soil bacterial community, polycyclic aromatic hydrocarbons, RAPD

Abstract

The RAPD (Random Amplified Polymorphic DNA) is an ease and inexpensive tool to analyze microorganism in the environment. However, the protocol optimization is a crucial step to provide a suitable polymorphic profile. In the present work, bacteria obtained from activated sludge system and soil impacted with industrial effluent were studied by RAPD technique to provide genetic similarities relationships. A similarity level of 91.9% to 56%, between isolated sludge and soil were observed. A genetic starter was selected after protocol optimization and a characteristic polymorphic bands profile generated to isolated microorganisms. The presence or absence of the isolated microorganisms in samples can be controlled by using the obtained polymorphic profile.

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Published

2018-04-23

Issue

Vol. 14 No. 1 (2015)

Section

Artigo

License

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How to Cite

Silva, R. G. da, Pinhati, F. R., & Silva, J. T. (2018). Analysis of genetic variability by RAPD of strains isolated from soil and sludge impacted with industrial effluent. Revista Da Biologia, 14(1), 1-5. https://doi.org/10.7594/revbio.14.01.01