A new technic to obtain a vaccine against avian spirochaetosis

Abstract

This paper reports a new embryonated egg technique in order to obtain a vaccine against avian spirochaetosis. Fowl-eggs about 10 days of incubation are infected in the allantois with 0.1 ml of citratated blood of a sick fowl. The survivant embryos on the sixth day are collected with fluids and membranes; the egg shells, vitelus and albumen are discarded. Collected material is then diluted 1:4 in glicerine-water 50% liquidified and passed through esteril gauze. The suspension is stored in a refrigerator and diluted 1:5 when necessary. This kind o f vaccine is proved active six months after production even when stored at room temperature, on the dosis of 1 ml injected by intramuscular route. The immunity is completed three days after inoculation. Some problems such modifications on biological properties and reproduction time of the Borrelia anserina are aborded.