Genetic transformation of Xanthomonas campestris to streptomicin and erythomycin resistence

Abstract

Evidence of transformation in Xanthomonas campestris was found established due to the fact DNA from streptomycin and erythromycin strains was able to transfer two genetic markers to sensitive a recipient strain. The cell competence, as established by the growth curve was located toward the end of the lag phase, but early in the exponential phase; the transformation rate of the streptomycin mark was about 2,2 x 10^-2 and 2%; for the erythromycin, it was about 2,5 x 10^-2 and 12,6%. For double transformants the figures were 1,6 x 10^-3 and 3,8 x 10^-2. No linkage was found between markers on the same molecule of DNA.