Factors influencing the determination of the proteic values of blood serum and plasma

Abstract

The authors study the influence of anticoagulants in blood samples taken for dosage of plasmatic proteins and their fractions, using sodium oxalate, in dry form and in solution, potassium oxalate in solution, Heller and Paul’s liquid, sodium citrate and EDTA. They study the influence of the same anticoagulants listed above in the determination of the total lipidic content and gamma-globulin. The influence of different concentrations of sodium sulphite and sodium sulphate used in the fractionization of proteins in emphasized, as well as the separation of globulins by filtration or by sulphuric ehter. The authors used sodium sulphite in the concentrations of 21%, 24%, 27% and sodium sulphate at 22,6%. They compare the results obtained in the dosage of albumin done by electrophoresis and by the method of Gornall et al., as well as the dosage of gamma-globulin obtained by electrophoresis and by the Frattini’s trubidimetric method. The authors present 8 tables with the results of their observations, discuss these results and present their conclusions.