Comparative analysis among efficiency of the male toad test, the ovarian hyperemia test and the augmentation of ovaries weight test in immature mice to precocious diagnosis of pregnancy in mares bred to a jack

Abstract

The following test was carried out with the blood serum samples drawn of 34 mares (between 30 and 114 days after the date of the last breeding): 94 male toad tests, 102 ovarian hyperemia tests and 55 augmentations of ovaries weight tests using immature mouse. Only negative results were obtained with the male toad tests; thus of 94 tests, 13 were in agreement with the clinical state of the animal (not pregnant) and 81 were not in agreement (false-negative reactions). Concerning the 102 ovarian hyperemia tests the results were: 21 in agreement (8 positives and 13 negatives) and 81 not in agreement (false-negative reactions) with the animals state. Finally of the 55 augmentation of ovaries weight tests a smaller number of false results was obtained: 8 false-negative results with blood serum samples obtained respectively in the 30^th, 31^st, 31^st, 31^st 33^rd, 89^th, 107^th days following the last breeding and 3 false-positive results with blood serum samples obtained in the 58^th, 74^th and 110^th; days after the last breeding; all samples belonged to the same mare. The statistical analysis of the results (Relative Risks Method) demonstrated that the capacity of the male toad test in providing results in agreement is, with 95% of probability hardly 0.227 (minimum 0.117 and maximum 0.442) times the capacity of the ovarian hyperemia and the augmentation of ovaries weight tests, while the capacity of the ovarian hyperemia test is 0.418 (minimum 0.234 and maximum 0.749) times the capacity of the augmentation of ovaries weight and the male toad tests in supply results in agreement with the clinical state of the animal. Finally, the augmentation of ovaries weight test is a parameter, 19.06 (minimum 8.933 maximum 40.636) times more efficient than the ovarian hyperemia and the male toad tests, for precocious diagnosis of pregnancy in the mare bred to a jack.