New change in the Brucella abortus differential staining technique

Authors

  • Elizabeth Oliveira da Costa Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Saemi Ogassawara Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Rolando Cury Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.2318-3659.v11i1p107-110

Keywords:

Technique, change, Differential staining, Brucella abortus

Abstract

A new modification of the differential stainning technique for B. abortus was described here. The method was based upon the well known resistance of B. abortus to alcali associated with heating during the staining of the smears. A fresh mixture of saturated aqueous solution of safranin and 1N potassium hydroxide was used in 1:2 proportion, respectively, for the clinical material and 4:1 for the bacterial cultures.

Downloads

Download data is not yet available.

Downloads

Published

1974-06-15

Issue

Vol. 11 No. 1 (1974)

Section

ORIGINAL ARTICLES

How to Cite

New change in the Brucella abortus differential staining technique. (1974). Revista Da Faculdade De Medicina Veterinária E Zootecnia Da Universidade De São Paulo, 11(1), 107-110. https://doi.org/10.11606/issn.2318-3659.v11i1p107-110