Indirect immunoflourescence rabies antibody technique for bovine serum. I — Technique adaptation

Abstract

The adaptation of the IFRA technique for bovine serum was consequent to the modification of the technique used in human serum, because the nervous cells membrane actuated as a barrier to the bovine antibodies. This difficult was eliminated by the treatment of the Amonn horn smears from dogs with rabies, with a hipotonic (0,15M) solution of sacharose during 30 minutes for 37°C. Others modifications were: the use of 40% CVS suspension for the antibodies neutralization and the fractionation of bovine normal serum through DEAE-CELLULOSE column.