Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência

Authors

  • Beatriz J. Celeste Universidade de São Paulo; Faculdade de Medicina; Instituto de Medicina Tropical de São Paulo; Seroepidemiology Laboratory
  • M. Carolina S. Guimarães Universidade de São Paulo; Faculdade de Medicina; Department of Preventive Medicine
  • Edelma Maria Corrales L. Universidade de São Paulo; Faculdade de Medicina; Instituto de Medicina Tropical de São Paulo; Seroepidemiology Laboratory

Keywords:

Mucocutaneous leishmaniasis serology, Performance index, Peroxidase antibody test, Fluorescent antibody test

Abstract

Performance indexes of the peroxidase antibody test were compared to that of the fluorescent antibody test. The peroxidase antibody test had a statistically higher sensitivity and negative predictive value and a higher efficiency than the fluorescent antibody test but its specificity and positive predictive value were within the 95% confidence limits for the values found for the fluorescent antibody test. Such differences did not change when Chagas' disease and visceral leishmaniasis sera were included in index calculations. Statistical analysis showed that the two tests have a substantial degree of agreement but the immunofluorescent test had a specificity index and a positive predictive value equal to 100.0% when Chagas' disease and visceral leishmaniasis sera were not included in the calculations of the performance index; in this instance, a positive test result equals a disclosure of the disease attribute due to the inexistence of false positive results. The enzyme/ protein ratio of the peroxidase conjugate, resulting in heavy or light-labeled conjugates may pose technical problems to its use in serology tests.

Downloads

Download data is not yet available.

Downloads

Published

1988-12-01

Issue

Section

Original Articles

How to Cite

Celeste, B. J., Guimarães, M. C. S., & Corrales L., E. M. (1988). Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência . Revista Do Instituto De Medicina Tropical De São Paulo, 30(6), 411-414. https://revistas.usp.br/rimtsp/article/view/28619