The immunoenzymatic assay (ELISA) in the serodiagnosis of Plasmodium vivax

Authors

  • Hilda A. Perez Instituto Venezolano de Investigaciones Científicas; Centro de Microbiología y Biología Celular; Laboratório de Inmunoparasitología
  • Mercedes De La Rosa Instituto Venezolano de Investigaciones Científicas; Centro de Microbiología y Biología Celular; Laboratório de Inmunoparasitología

Keywords:

Malaria, P. vivax, Anticuerpos, ELISA

Abstract

ELISA was evaluated for the serodiagnosis of Plasmodium vivax using homologous antigen. This was a crude fraction obtained after detergent (NP-40) lysis of human parasitized red blood cells. Antibodies of the classes IgM, IgG, IgA were determined in a pool of eleven sera from patients with P. vivax malaria. The protein A was introduced as secondary probe to screen P. vivax antibodies in 30 sera of patients harbouring a first episode of P. vivax malaria. There was a correlation of 93% with the parasitological diagnosis and the test resulted specific and reproducible.

Downloads

Download data is not yet available.

Downloads

Published

1990-06-01

Issue

Vol. 32 No. 3 (1990)

Section

Serology

How to Cite

Perez, H. A., & De La Rosa, M. (1990). The immunoenzymatic assay (ELISA) in the serodiagnosis of Plasmodium vivax . Revista Do Instituto De Medicina Tropical De São Paulo, 32(3), 189-195. https://revistas.usp.br/rimtsp/article/view/28731