Entamoeba histolytica: antigenic characterization of axenic strains from Brazil

Authors

  • Semíramis Guimarães Federal Minas Gerais University; Institute of Biological Science; Department of Parasitology
  • Haidée Urdaneta Los Andes University; Department of Biology
  • Edward F. Silva Federal Minas Gerais University; Institute of Biological Science; Department of Parasitology
  • Carlos Alberto P. Tavares Federal Minas Gerais University; Institute of Biological Science; Department of Biochemistry and Immunology

Keywords:

Entamoeba histolytica, Axenic strains, Antigens

Abstract

Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.

Downloads

Download data is not yet available.

Downloads

Published

1991-02-01

Issue

Vol. 33 No. 1 (1991)

Section

Original Article

How to Cite

Guimarães, S., Urdaneta, H., Silva, E. F., & Tavares, C. A. P. (1991). Entamoeba histolytica: antigenic characterization of axenic strains from Brazil . Revista Do Instituto De Medicina Tropical De São Paulo, 33(1), 6-11. https://revistas.usp.br/rimtsp/article/view/28784