Attempts for breeding triatominae in laboratory by means of "in vitro" feeding

Authors

  • Hertha Barbara Wüllert Telles de Souza Laboratório de Investigação Médica-Parasitologia
  • Vicente Amato Neto Laboratório de Investigação Médica-Parasitologia
  • Lucia Maria Almeida Braz Laboratório de Investigação Médica-Parasitologia
  • Antonio Augusto Baillot Moreira Laboratório de Investigação Médica-Parasitologia
  • Clovis Kiomitsu Takiguti Universidade de São Paulo; Faculdade de Medicina; Departamento de Medicina Preventiva
  • Rubens Campos Laboratório de Investigação Médica-Parasitologia
  • Luís Matsubara Laboratório de Investigação Médica-Parasitologia

Keywords:

Criação de triatomíneos, Alimentação "in vitro", Desenvolvimento, Infecção pelo Trypanosoma cruzi

Abstract

In order to simplify breeding of triatominae in the laboratory, for performing xenodiagnosis and other biologic studies, we tried to feed the insects "in vitro" with citrated or defibrinated blood from commercially abated chicken. Two types of efficacy observations were carried out with Triatoma infestans: a) analysis of the chaining of successive nymphal stages, viability of satisfactory matching, fertile oviposition and adequate reproduction; b) assessment of viability and infectivity of Trypanosoma cruzi in the insects. As a conclusion, it became evidente that, despite operational easiness, the objectives were not achieved, since the classical procedure used as the control, was always superior.

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Published

1991-06-01

Issue

Section

Technical Assays

How to Cite

Souza, H. B. W. T. de, Amato Neto, V., Braz, L. M. A., Moreira, A. A. B., Takiguti, C. K., Campos, R., & Matsubara, L. (1991). Attempts for breeding triatominae in laboratory by means of "in vitro" feeding . Revista Do Instituto De Medicina Tropical De São Paulo, 33(3), 221-226. https://revistas.usp.br/rimtsp/article/view/28823