Isolation and characterization of partially purified leptospiral antigens

Authors

  • M.C. Koury UFMg; ICB; Department of Microbiology
  • H.A. Rangel Unicamp; Department of Microbiology and Immunology

Keywords:

Leptospira, Antigens, Purification, Diagnosis

Abstract

The methanol extract of Leptospira interrogans serovar canicola was purified by precipitation with acetone or acetone and chloroform. The antigenicity of the antigen was not altered by heating or treatment with pepsin and pronase. However the antigenicity was lost when the antigen was treated with periodic acid. Chemical analysis revealed the presence of 40% carbohydrate (22% methylpentose, 28%; hexoses),4% protein, 20% lipid and 2,7% phosphate. The complement fixation test with sera from patients with leptospirosis agreed with the microscopic agglutination reaction.

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Published

1991-10-01

Issue

Section

Immunology

How to Cite

Koury, M., & Rangel, H. (1991). Isolation and characterization of partially purified leptospiral antigens . Revista Do Instituto De Medicina Tropical De São Paulo, 33(5), 391-396. https://revistas.usp.br/rimtsp/article/view/28859