Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples

Authors

  • Jose Laferte Instituto de Medicina Tropical Pedro Kouri; Departamento de Virología
  • Miguel Marrero Instituto de Medicina Tropical Pedro Kouri; Departamento de Virología
  • Maritza Alvarez Instituto de Medicina Tropical Pedro Kouri; Departamento de Virología
  • Liliana Jomarron Instituto de Medicina Tropical Pedro Kouri; Departamento de Virología
  • Serafina Garcia Instituto de Medicina Tropical Pedro Kouri; Departamento de Virología
  • Susana Vazquez Instituto de Medicina Tropical Pedro Kouri; Departamento de Virología
  • Luis Morier Instituto de Medicina Tropical Pedro Kouri; Departamento de Virología
  • Mario Ulacia Banco de sangre provincial
  • Antonio Melchor Centro de Inmunoensayo

Keywords:

UltramicroELISA, Citomegalovirus

Abstract

We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (1:40) and anti-human conjugate working dilution (1:1500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indiret immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.

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Published

1992-02-01

Issue

Section

Serology

How to Cite

Laferte, J., Marrero, M., Alvarez, M., Jomarron, L., Garcia, S., Vazquez, S., Morier, L., Ulacia, M., & Melchor, A. (1992). Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples . Revista Do Instituto De Medicina Tropical De São Paulo, 34(1), 43-47. https://revistas.usp.br/rimtsp/article/view/28890