UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots

Authors

  • Adriana Torrella Centro de Inmunoensayo
  • Rosa L. Solis Centro de Inmunoensayo
  • Nerys Rodriguez Centro de Inmunoensayo
  • Yadira Medina Hospital Especializado Dermatológico
  • Martha Pita Hospital Especializado Dermatológico
  • Ivis Perez Hospital Especializado Dermatológico
  • Nubia Licourt Hospital Especializado Dermatológico

Keywords:

Lepra, IgM anti-glicolipido fenolico-I (IgM ANTI GF-I) UltramicroELISA, Sangre seca en papel de filtro

Abstract

In this work the adequate conditions for the detection of IgM antibodies to PGL-I in eluates from dried blood spots using the UMELISA HANSEN and the SUMA technology are established. A total of 300 blood donors and 58 leprosy patients samples were studied. For both populations, in the previously established conditions, we compared the results of the eluates from dried blood spots with the serum samples, and was obtained a correlation of 0.919 in blood donors, 0.969 in patients, and 0.954 for the total of both populations. It was also obtained a level of agreemnet of 98% in patients and 96% in blood donors. In the patients population was found a sensitivity of 93% and an specificity of 100% for the eluates of dried blood spots evaluated by the UMELISA HANSEN in the comparison with the serum samples analized by the same assay.

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Published

1994-04-01

Issue

Vol. 36 No. 2 (1994)

Section

Serology

How to Cite

Torrella, A., Solis, R. L., Rodriguez, N., Medina, Y., Pita, M., Perez, I., & Licourt, N. (1994). UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots . Revista Do Instituto De Medicina Tropical De São Paulo, 36(2), 131-138. https://revistas.usp.br/rimtsp/article/view/29136