Evaluation of different methods for Plasmodia detection, in well defined population groups in an endemic area of Brazil

Authors

  • Sandra L. M. Avila University of São Paulo; Institute of Tropical Medicine of São Paulo
  • Mirlan C. Leandro University of São Paulo; Institute of Tropical Medicine of São Paulo
  • Noemia B. Carvalho University of São Paulo; Infectious Diseases Department
  • Marilia S. Oliveira University of São Paulo; Infectious Diseases Department
  • Viviana G. Arruk University of São Paulo; Institute of Tropical Medicine of São Paulo
  • Maria Carmen A. Sanchez University of São Paulo; Institute of Tropical Medicine of São Paulo
  • Marcos Boulos University of São Paulo; Infectious Diseases Department
  • Antonio Walter Ferreira University of São Paulo; Infectious Diseases Department

Keywords:

Malaria, Plasmodia detection, diagnosis, IFA, QBC

Abstract

In Brazil, more than 500,000 new cases of malaria were notified in 1992. Plasmodium falciparum and P.vivax are the responsible species for 99.3% of the cases. For adequate treatment, precoce diagnosis is necessary. In this work, we present the results of the traditional Plasmodia detection method, thick blood film (TBF), and the results of alternative methods: Immunofluorescence assay (IFA) with polyclonal antibody and Quantitative Buffy Coat method (QBC)® in a well defined population groups. The analysis were done in relation to the presence or absence of malaria clinical symptoms. Also different classes of immunoglobulins anti-P.falciparum were quantified for the global analysis of the results, mainly in the discrepant results. We concluded that alternative methods are more sensitive than TBF and that the association of epidemiological, clinical and laboratory findings is necessary to define the presence of malaria.

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Published

1994-04-01

Issue

Section

Technical Trial

How to Cite

Avila, S. L. M., Leandro, M. C., Carvalho, N. B., Oliveira, M. S., Arruk, V. G., Sanchez, M. C. A., Boulos, M., & Ferreira, A. W. (1994). Evaluation of different methods for Plasmodia detection, in well defined population groups in an endemic area of Brazil . Revista Do Instituto De Medicina Tropical De São Paulo, 36(2), 157-162. https://revistas.usp.br/rimtsp/article/view/29140