Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories

Authors

  • Ana Maria Díaz Instituto Panamericano de Protección de Alimentos y Zoonosis
  • Elizabeth Juliana Ghiuro Valentini Instituto Butantã
  • Avelino Albas Instituto Butantã
  • Regina Maria Mourão Fuches Instituto Butantã
  • Neuza Maria Frazatti Gallina Instituto Butantã

Keywords:

Antirabies sera, Counterimmunoelectrophoresis, Standard Mouse Neutralization Test

Abstract

To determine the rabies antibody level of twenty-four hyperimmune equine sera, Standard Mouse Neutralization (SMN) and Couterimmunoelectrophoresis (CIE) tests were carried out, both at the Instituto Butantan (IB) and Instituto Panamericano de Protección de Alimentos y Zoonosis (INPPAZ). Statistical analysis has shown a correlation (r) of 0.9317 between the SMN and CIE performed at the IB, while at the INPPAZ it scored 0.974. Comparison of CIE data of both laboratories yielded a correlation of 0.845. The CIE technique has shown to be as sensitive and efficient as the SMN in titrating antirabies hyperimmune equine sera. Based on CIE results, a simple, rapid and inexpensive technique, tilers of sera antibody can be reliably estimated in SMN test.

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Published

1995-10-01

Issue

Section

Serology

How to Cite

Díaz, A. M., Valentini, E. J. G., Albas, A., Fuches, R. M. M., & Gallina, N. M. F. (1995). Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories . Revista Do Instituto De Medicina Tropical De São Paulo, 37(5), 435-440. https://revistas.usp.br/rimtsp/article/view/29304