A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp

Authors

  • Francisco Hernández University of Costa Rica; Faculty of Microbiology

Keywords:

Campylobacter, technical assay, Microaerobiosis^i1^spoul

Abstract

Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.

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Published

1996-06-01

Issue

Section

Technical Trial

How to Cite

Hernández, F. (1996). A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp . Revista Do Instituto De Medicina Tropical De São Paulo, 38(3), 241-242. https://revistas.usp.br/rimtsp/article/view/29370