Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens

Authors

  • Noeli M. ESPÍNDOLA Universidade de São Paulo; Faculdade de Ciências Farmacêuticas
  • Elizabeth N. DE GASPARI Instituto Adolfo Lutz
  • Paulo M. NAKAMURA Instituto Adolfo Lutz
  • Adelaide J. VAZ Universidade de São Paulo; Faculdade de Ciências Farmacêuticas

Keywords:

Taenia sol, Taenia crassic, Monoclonal antib, Cysticerco

Abstract

We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.

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Published

2000-06-01

Issue

Vol. 42 No. 3 (2000)

Section

Brief Communication

How to Cite

ESPÍNDOLA, N. M., DE GASPARI, E. N., NAKAMURA, P. M., & VAZ, A. J. (2000). Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens . Revista Do Instituto De Medicina Tropical De São Paulo, 42(3), 175-177. https://revistas.usp.br/rimtsp/article/view/30432