Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

Authors

  • Liana Konovaloff JANNOTTI-PASSOS FIOCRUZ; Centro de Pesquisas René Rachou; Laboratório de Malacologia
  • Cecilia Pereira de SOUZA FIOCRUZ; Centro de Pesquisas René Rachou; Laboratório de Malacologia

Keywords:

Schistosoma mans, Biomphalaria tenagoph, Biomphalaria strami, Susceptibil, LS-PCR techni

Abstract

In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.

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Published

2000-10-01

Issue

Section

Brief Communication

How to Cite

JANNOTTI-PASSOS, L. K., & SOUZA, C. P. de. (2000). Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction . Revista Do Instituto De Medicina Tropical De São Paulo, 42(5), 291-294. https://revistas.usp.br/rimtsp/article/view/30458