Evaluation of Etest and macrodilution broth method for antifungal susceptibility testing of Candida sp strains isolated from oral cavities of AIDS patients

Authors

  • Maria do Rosário R. SILVA Universidade Federal de Goiás; Instituto de Patologia Tropical e Saúde Pública
  • Márcio R. COSTA Universidade Federal de Goiás; Instituto de Patologia Tropical e Saúde Pública
  • André T.B. MIRANDA Universidade Federal de Goiás; Instituto de Patologia Tropical e Saúde Pública
  • Orionalda de F.L. FERNANDES Universidade Federal de Goiás; Instituto de Patologia Tropical e Saúde Pública
  • Carolina R. COSTA Universidade Federal de Goiás; Instituto de Patologia Tropical e Saúde Pública
  • Claudete R. de PAULA Universidade de São Paulo; Instituto de Ciências Biomédicas

Keywords:

Etest, Broth macrodilution, Antifungal susceptibility tests

Abstract

A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at ± 2 dilutions for both methods were considered, the agreement rates were 90.4% for itraconazole, ketoconazole and amphotericin B and 84.6% for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories.

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Published

2002-01-01

Issue

Section

Mycology

How to Cite

SILVA, M. do R. R., COSTA, M. R., MIRANDA, A. T., FERNANDES, O. de F., COSTA, C. R., & PAULA, C. R. de. (2002). Evaluation of Etest and macrodilution broth method for antifungal susceptibility testing of Candida sp strains isolated from oral cavities of AIDS patients . Revista Do Instituto De Medicina Tropical De São Paulo, 44(3), 121-125. https://revistas.usp.br/rimtsp/article/view/30571