Staphylococcus aureus ampicillin-resistant from the odontological clinic environment

Authors

  • Wagner Luis de Carvalho Bernardo Universidade Estadual de Campinas; Faculdade de Odontologia de Piracicaba; Laboratório de Microbiologia e Imunologia; Departamento de Diagnóstico Oral
  • Marcelo Fabiano Gomes Boriollo Universidade Estadual de Campinas; Faculdade de Odontologia de Piracicaba; Laboratório de Microbiologia e Imunologia; Departamento de Diagnóstico Oral
  • Reginaldo Bruno Gonçalves Universidade Estadual de Campinas; Faculdade de Odontologia de Piracicaba; Laboratório de Microbiologia e Imunologia; Departamento de Diagnóstico Oral
  • José Francisco Höfling Universidade Estadual de Campinas; Faculdade de Odontologia de Piracicaba; Laboratório de Microbiologia e Imunologia; Departamento de Diagnóstico Oral

Keywords:

Staphylococcus aureus, Ampicillin-resistant, Odontological clinic environment

Abstract

The aim of this research was to evaluate the prevalence of Sthaphylococcus spp. and S. aureus in the odontological clinic environment (air), their production of beta-lactamase and antibacterial susceptibility to the major antibiotics utilized in medical particle. During 12 months of samples collect were isolated 9775 CFU by MSA medium suggesting a high amount of Staphylococcus spp. in the clinic environment which can appear through aerosols. A total of 3149 colonies (32.2%) were suggestive of pathogenic staphylococci. Gram coloration, catalase test, colony-mallow growing on chromogenic medium, and coagulase test confirmed the identity of 44 (0.45%) S. aureus isolates. Of these, 35 isolates (79.5%) showed production of beta-lactamase by CefinaseTM discs and resistance to ampicillin, erythromycin (7 isolates) and tetracycline (1 isolate) suggesting the existence of multiresistant isolates. The evaluation of the oxacillin MIC by Etest® assays showed susceptibility patterns suggesting the inexistence of the mecA gene in chromosomal DNA. These results point out to the need of a larger knowledge on the contamination means and propagation of this microorganism into the odontological clinic.

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Published

2005-02-01

Issue

Section

Microbiology

How to Cite

Bernardo, W. L. de C., Boriollo, M. F. G., Gonçalves, R. B., & Höfling, J. F. (2005). Staphylococcus aureus ampicillin-resistant from the odontological clinic environment . Revista Do Instituto De Medicina Tropical De São Paulo, 47(1), 19-24. https://revistas.usp.br/rimtsp/article/view/30879