Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil

Authors

  • Pedro Alves d'Azevedo Fundação Faculdade Federal de Ciências Médicas de Porto Alegre; Departamento de Microbiologia e Parasitologia
  • Cícero Armídio Gomes Dias Fundação Faculdade Federal de Ciências Médicas de Porto Alegre; Departamento de Microbiologia e Parasitologia
  • Lúcia Martins Teixeira Universidade Federal do Rio de Janeiro; Instituto de Microbiologia Prof. Paulo de Góes

Keywords:

Enterococci, High level resistance aminoglycosides, Genetic diversity

Abstract

In the present study, a total of 455 enterococcal isolates, recovered from patients living in the city of Porto Alegre, State of Rio Grande do Sul, Brazil, during the period from July 1996 to June 1997, were identified to the species level by conventional biochemical and microbiological tests, and assayed for their susceptibilities to antimicrobial agents. The genetic diversity of antimicrobial resistant strains was evaluated by pulsed-field gel electrophoresis (PFGE) analysis of SmaI restricted chromosomal DNA. The most frequent species was Enterococcus faecalis (92.8%). Other species identified were: E. faecium (2.9%), E. gallinarum (1.5%), E. avium (1.1%), E. hirae (0.7%), E. casseliflavus (0.4%), E. durans (0.4%) and E. raffinosus (0.2%). The overall prevalence of isolates with high-level resistance (HLR) to aminoglycosides was 37.8%. HLR to gentamicin was found in 24.8%. No strains with acquired resistance to vancomycin were found. PFGE analysis showed the predominance of clonal group A, comprising strains isolated from different clinical specimens obtained from patients in three hospitals. These results suggest intra and inter-hospital dissemination of one predominant clonal group of E. faecalis isolates with HLR to gentamicin in the hospitals included in this study.

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Published

2006-02-01

Issue

Section

Microbiology

How to Cite

d'Azevedo, P. A., Dias, C. A. G., & Teixeira, L. M. (2006). Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil . Revista Do Instituto De Medicina Tropical De São Paulo, 48(1), 11-16. https://revistas.usp.br/rimtsp/article/view/30961