An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model

Authors

  • Sílvia Regina Ferreira Gonçalves Pereira Universidade Estadual do Norte Fluminense Darcy Ribeiro; Centro de Ciências e Tecnologias Agropecuárias; Setor de Virologia e Viroses; Laboratório de Sanidade Animal
  • Fernando Portela Câmara Universidade Federal do Rio de Janeiro; Instituto de Microbiologia Professor Paulo de Góes; Centro de Ciências da Saúde; Setor de Epidemiologia de Doenças Infecciosas
  • Maria Angélica Arpon Marandino Guimarães Universidade Federal do Rio de Janeiro; Instituto de Microbiologia Professor Paulo de Góes; Centro de Ciências da Saúde; Departamento de Virologia
  • Leonardo Vieira Neto Universidade Federal do Rio de Janeiro; Instituto de Microbiologia Professor Paulo de Góes; Centro de Ciências da Saúde; Departamento de Virologia
  • Daniel Segenreich Universidade Federal do Rio de Janeiro; Instituto de Microbiologia Professor Paulo de Góes; Centro de Ciências da Saúde; Departamento de Virologia
  • Antônio Carlos da Costa Guimarães Universidade Federal do Rio de Janeiro; Instituto de Microbiologia Professor Paulo de Góes; Centro de Ciências da Saúde; Departamento de Virologia
  • Vera Lucia Antunes Chagas Universidade Federal do Rio de Janeiro; Hospital Universitário Clementino Fraga Filho; Departamento de Patologia

Keywords:

Herpes simplex virus type 1, Ophthalmic herpes, Murine model, Monoclonal antibodies, Indirect immunofluorescence

Abstract

Herpes simplex virus type 1 (HSV-1) ophthalmic disease is the most common cause of corneal blindness in humans world-wide. Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evaluate the use of a quicker and simpler method to herpes ophthalmic diagnosis. Corneal smears were made by firm imprints of infected mouse eyes to glass slides, after smears were fixated with cold acetone, and an indirect immunofluorescence (IIF) method was performed using monoclonal antibodies in a murine model of ophthalmic herpes. Eye swabs from infected mice were inoculated in Vero cells for virus isolation. Cytology and histology of the eye were also performed, using hematoxylin-eosin routine. Mouse eyes were examined by slit-lamp biomicroscopy for evidence of herpetic disease at various times postinoculation. We made a comparative evaluation of sensitivity, specificity and speed of methods for laboratory detection of HSV. Our results indicate that this IIF method is quick, sensitive, specific and can be useful in the diagnosis of ophthalmic herpes as demonstrated in an animal model.

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Published

2007-04-01

Issue

Section

Immunofluorescence

How to Cite

Pereira, S. R. F. G., Câmara, F. P., Guimarães, M. A. A. M., Vieira Neto, L., Segenreich, D., Guimarães, A. C. da C., & Chagas, V. L. A. (2007). An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model . Revista Do Instituto De Medicina Tropical De São Paulo, 49(2), 87-92. https://revistas.usp.br/rimtsp/article/view/31066