Tnf-a production and apoptosis in hepatocytes after listeria monocytogenes and salmonella typhimurium invasion
Keywords:
Listeria monocytogenes, Salmonella Typhimurium, Hepatocytes, Apoptosis, Tumor necrosis factor-alphaAbstract
Invasion of hepatocytes by Listeria monocytogenes (LM) and Salmonella Typhimurium (ST) can stimulate tumor necrosis factor alpha (TNF-α) release and induce apoptosis. In this study, we compared the behavior of hepatocytes invaded by three L. monocytogenes serotypes (LM-4a, LM-4b and LM-1/2a) and by ST to understand which bacterium is more effective in the infectious process. We quantified TNF-α release by ELISA, apoptosis rates by annexin V (early apoptosis) and TUNEL (late apoptosis) techniques. The cell morphology was studied too. TNF-α release rate was highest in ST-invaded hepatocytes. ST and LM-1/2a induced the highest apoptosis production rates evaluated by TUNEL. LM-4b produced the highest apoptosis rate measured by annexin. Invaded hepatocytes presented various morphological alterations. Overall, LM-4b and LM-1/2a proved to be the most efficient at cell invasion, although ST adapted faster to the environment and induced earlier hepatocyte TNF-α release.Downloads
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Published
2011-04-01
Issue
Section
Microbiology
How to Cite
Santos, S. A. D., Andrade Júnior, D. R. D., & Andrade, D. R. D. (2011). Tnf-a production and apoptosis in hepatocytes after listeria monocytogenes and salmonella typhimurium invasion . Revista Do Instituto De Medicina Tropical De São Paulo, 53(2), 107-112. https://revistas.usp.br/rimtsp/article/view/31385