SEROLOGICAL AND MOLECULAR SURVEY OF Leptospira spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL

Authors

  • Mariane Angélica Finger Federal University of Paraná (UFPR); Department of Veterinary Medicine
  • Ivan Roque de Barros Filho Federal University of Paraná (UFPR); Department of Veterinary Medicine
  • Christian Leutenegger Idexx Reference Laboratories; Real Time PCR Core Facility
  • Marko Estrada Idexx Reference Laboratories; Real Time PCR Core Facility
  • Leila Sabrina Ullmann São Paulo State University; Veterinary School; Department of Veterinary Hygiene and Public Health
  • Hélio Langoni São Paulo State University; Veterinary School; Department of Veterinary Hygiene and Public Health
  • Mariana Kikuti São Paulo State University; Veterinary School; Department of Veterinary Hygiene and Public Health
  • Peterson Triches Dornbush Federal University of Paraná (UFPR); Department of Veterinary Medicine
  • Ivan Deconto Federal University of Paraná (UFPR); Department of Veterinary Medicine
  • Alexander Welker Biondo University of Illinois; Department of Pathobiology

Abstract

Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.

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Published

2014-12-01

Issue

Vol. 56 No. 6 (2014)

Section

Leptospirosis

How to Cite

Finger, M. A., Barros Filho, I. R. de, Leutenegger, C., Estrada, M., Ullmann, L. S., Langoni, H., Kikuti, M., Dornbush, P. T., Deconto, I., & Biondo, A. W. (2014). SEROLOGICAL AND MOLECULAR SURVEY OF Leptospira spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL. Revista Do Instituto De Medicina Tropical De São Paulo, 56(6), 473-476. https://revistas.usp.br/rimtsp/article/view/87657