Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers

Authors

  • Christiane Moreira Padovani University of São Paulo; School of Nursing
  • Kazuko Uchikawa Graziano University of São Paulo; School of Nursing
  • Vânia Regina Goveia University of São Paulo; School of Nursing

DOI:

https://doi.org/10.1590/S0104-11692008000600016

Keywords:

anti-infective agents, local, contamination, cross infection

Abstract

This study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (PVP-I) and chlorhexidine (CHX) - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. A laboratory study was performed with 180 antiseptic containers, which were contaminated with Serratia marcescens [1 x 105 UFC/mL]. The containers were closed and stored, at room temperature, during seven days and shaken daily. The antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the non-disposable containers. These results suggested that antiseptic solutions inactivate microorganisms [1 x 105 UFC/mL]. Since cleaned antiseptic containers have around 102 UFC coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVP-I solutions in aqueous, detergent and alcoholic formulations.

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Published

2008-12-01

Issue

Vol. 16 No. 6 (2008)

Section

Original Articles

License

RLAE’s authorship concept is based on the substantial contribution by each of the individuals listed as authors, mainly in terms of conceiving and planning the research project, collecting or analyzing and interpreting data, writing and critical review. Indication of authors’ names under the article title is limited to six. If more, authors are listed on the online submission form under Acknowledgements. The possibility of including more than six authors will only be examined on multicenter studies, considering the explanations presented by the authors.
Including names of authors whose contribution does not fit into the above criteria cannot be justified. Those names can be included in the Acknowledgements section.
 Authors are fully responsible for the concepts disseminated in their manuscripts, which do not necessarily reflect the editors’ and editorial board’s opinion.

 

How to Cite

Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers. (2008). Revista Latino-Americana De Enfermagem, 16(6), 1038-1041. https://doi.org/10.1590/S0104-11692008000600016