The ferric aerobactin receptor IutA, a protein isolated on agarose column, is not essential for uropathogenic Escherichia coli infection

Authors

  • Taise Natali Landgraf Universidade de São Paulo; Faculdade de Medicina de Ribeirão Preto
  • Alan Berlese Universidade de São Paulo; Faculdade de Medicina de Ribeirão Preto
  • Fabricio Freitas Fernandes Universidade de São Paulo; Faculdade de Medicina de Ribeirão Preto
  • Mariani Lima Milanezi Universidade de São Paulo; Faculdade de Medicina de Ribeirão Preto
  • Roberto Martinez Universidade de São Paulo; Faculdade de Medicina de Ribeirão Preto
  • Ademilson Panunto-Castelo Universidade de São Paulo; Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto

DOI:

https://doi.org/10.1590/S0104-11692012000200017

Keywords:

Uropathogenic Escherichia coli, Siderophores, Virulence

Abstract

Although many proteins have been described involved in Escherichia coli colonization and infection, only few reports have shown lectins as important components in these processes. Because the mechanisms underlying E. coli colonization process involving lectins are not fully understood, we sought to identify the presence of other non-described lectins in E. coli. Here, we isolated a 75-kDa protein from E. coli on Sepharose column and identified it as ferric aerobactin receptor (IutA). Since IutA is controversially associated with virulence of some E. coli strains, mainly in uropathogenic E. coli (UPEC), we evaluated the presence of iutA gene in UPEC isolated from patients with urinary infection. This gene was present in only 38% of the isolates, suggesting a weak association with virulence. Because there is a redundancy in the siderophore-mediated uptake systems, we suggest that IutA can be advantageous but not essential for UPEC.

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Published

2012-04-01

Issue

Vol. 20 No. 2 (2012)

Section

Original Articles

License

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Including names of authors whose contribution does not fit into the above criteria cannot be justified. Those names can be included in the Acknowledgements section.
 Authors are fully responsible for the concepts disseminated in their manuscripts, which do not necessarily reflect the editors’ and editorial board’s opinion.

 

How to Cite

The ferric aerobactin receptor IutA, a protein isolated on agarose column, is not essential for uropathogenic Escherichia coli infection. (2012). Revista Latino-Americana De Enfermagem, 20(2), 340-345. https://doi.org/10.1590/S0104-11692012000200017