Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
DOI:
https://doi.org/10.1590/S0103-90161995000300023Keywords:
Metarhizium anisopliae, auxotrophk mutants, colour mutants, mutagenic agents, gamma radiation, ultraviolet light, nitrous acidAbstract
A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.Downloads
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Published
1995-12-01
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All content of the journal, except where identified, is licensed under a Creative Common attribution-type BY-NC.How to Cite
Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae . (1995). Scientia Agricola, 52(3), 548-554. https://doi.org/10.1590/S0103-90161995000300023