Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping
DOI:
https://doi.org/10.1590/1678-992x-2016-0419Palabras clave:
PCR, ectomycorrhizal fungi, molecular identification, morphotypeResumen
Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrients and water absorption, as well as protection of the host plant against pathogens and abiotic stresses. Most ectomycorrhizal fungi belong to the Basidiomycota class, such as the following genera: Amanita, Hebeloma, Hysterangium, Laccaria, Lactarius, Rhizopogon, Russula, Scleroderma, Suillus, Tricholoma, among others. Morphological studies on ectomycorrhizae report important results in understanding the species biodiversity. However, the use of molecular biology nowadays is indispensable. Among the various molecular tools available, there is consensus about the use of tools based on sequencing of the Internal Transcribed Spacer (ITS) of fungi rDNA, aiding in species characterization and construction of phylogenetic studies. The ITS region is of easy amplification, it has multicopy nature and enables differentiation between species. The objective of this study was to show that the use of molecular biology tools associated with morphotyping to characterize species of ectomycorrhizae is more effective than when they are used on their own.Descargas
Los datos de descarga aún no están disponibles.
Descargas
Publicado
2018-05-01
Número
Sección
Review
Licencia
Todo el contenido de la revista, excepto donde esté identificado, está protegido por el Creative Commons del tipo BY-NCCómo citar
Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping. (2018). Scientia Agricola, 75(3), 246-254. https://doi.org/10.1590/1678-992x-2016-0419